中文摘要：

目的建立神经病理性痛大鼠脊髓蛋白质组图谱。方法成年雄性SD大鼠16只，体重260～320g，随机分为2组（n=8）：假手术组（S组）和神经病理性痛组（NP组）。采用慢性缩窄性坐骨神经损伤法（CCI）制备大鼠神经病理性痛模型，S组仅暴露坐骨神经，不结扎。分别于CCI前1d（基础状态）及CCI后5、7d测定机械痛阈和热痛阈；于CCI后7d痛阈测定结束后取腰段脊髓组织，每组大鼠的脊髓组织混合后提取总蛋白质，测定脊髓组织蛋白质含量。以固相pH值梯度等电聚焦（IEF）为第一向，SDS—PAGE垂直电泳为第二向进行双向凝胶电泳（2-DE），制备脊髓蛋白质2-DE图谱，应用图像分析软件分析2-DE图谱，并观察其差异表达情况。结果与S组比较，NP组CCI后机械痛闽和热痛阈降低（P〈0．05）；两组大鼠脊髓组织总蛋白质经双向凝胶电泳分离，银染显色后得到分辨率高、重复性好的2-DE图谱，蛋白质点主要分布在pH3～10，相对分子质量为8～120的区域；NP组差异表达的蛋白质有23个，其中16个蛋白质表达上调，7个蛋白质表达下调（P〈0．05）；表达上调幅度最大的为SSP2203蛋白，表达下调幅度最大的为SSP1807蛋白（P〈0．05）。结论本研究成功地建立了神经病理性痛大鼠脊髓蛋白质组的图谱。

英文摘要：

Objective To establish the proteomics of the spinal cord in rats with neuropathic pain. Methods Sixteen adult male SD rats weighing 260-320 g were randomly divided into 2 groups （ n = 8 each） ： sham operation group （S） and neuropathic pain group （NP）. Neuropathic pain was induced by chronic constrictive injury （CCI）. The animals were anesthetized with intraperitoneal （IP） 10% chloral hydrate 300 mg/kg. The right sciatic nerve was exposed and 4 ligatures were placed on the sciatic nerve at 1 mm intervals. Theshold to thermal and mechanical stimulation was measured 1 day before and at 5, 7 d after CCI. The animals were sacrificed at 7 d after pain threshold measurement. The lumbar enlargement segments of the spinal cord were harvested and proteins resolved by 2-DE. Differential proteome profiles were established and analyzed by means of immobilized pH gradient based on two-dimensional polyacrylamide gel electrophoresis （2D-PAGE）. The 2-DE maps were visualized after silver staining and analyzed with image Master 2-DE software. Results There were 546 and 532 spots obtained in 2-DE maps of rat spinal cord tissue in group S and NP, and 23 proteins were found to be differentially expressed in NP group as compared with S group. Of the 23 proteins the expression of the 17 spots was increased and 6 spots decreased in quantity. Conclusion The optimized 2-DE profiles of proteome from the spinal cord of the rats with neuropathic pain were established preliminarily. It lays foundation for the proteome research of neuropathic pain.