针对焦化废水污染现状,采用水培试验,研究了不同体积浓度焦化废水对玉米幼苗生长、生物量、抗氧化酶活性及非酶类抗氧化物质含量的影响,以期为焦化废水的毒性检测及生物监控指标的确定提供理论依据。结果表明,焦化废水经过A2/O工艺处理后,仍然能够显著抑制玉米幼苗的生长和生物量,且该抑制作用表现为对处理浓度和作用时间的双重依赖,处理浓度增大或作用时间延长对玉米幼苗生长和生物量的抑制作用增强;同时,经焦化废水处理后,染毒组玉米幼苗抗坏血酸(AsA)含量和抗坏血酸过氧化物酶(APX)活性明显增高,而谷胱甘肽(GSH)含量和谷胱甘肽过氧化物酶(GPX)活性却降低。这一结果提示,焦化废水经过处理后,CODCr、BOD5、NH3-N、挥发酚、氰化物、硫化物、油等物理化学指标大幅降低,但其生态风险依然存在。
Coking wastewater pollution has become one major of various environmental problems. To provide theoretical basis for toxicity testing and biological monitoring indexes of coking wastewater, maize seedlings were exposed to different volumn concentrations of coking wastewater(50%, 80%, 100% ), then its effects on the growth, fresh biomass, activities of antioxidant enzymes(ascorbate and glutathione peroxidase ) and non-enzymatic antioxidants(ascorbate and glutathione ) content in maize seedlings(Zea mays L. ) were investigated with a hydroponic experiment. Compared with the control group, significant reduction in root and shoot length was observed in a time and concentration-dependent manner at all concentrations tested at both 5 and 7 days, and furthermore, reduction in root and shoot fresh biomass was observed. Meanwhile, coking wastewater caused a more pronounced increase in ascorbate (AsA) content ( 122. 1% of control) and activity of ascorbate peroxidase ( APX ) ( 117.5% of control ), whereas decreased glutathione ( GSH ) content( 73.9% of control ) and activity of glutathione peroxidase( GPX )( 81.2% of control ) after 30% sample exposure in 8-day-old seedlings. It was concluded that, conventional treatment could significantly decreased COD6, BOD5, NH3-N, volatile phenols, cyanide, sulfide and oil value of coking wastewater, but still some hazardous things remained in effluent which could restrain plant growth and fresh biomass, resulting in phytotoxicity. In addition, the inhibition depended on the effluent concentration and the treatment time, and also pollution risk mainly focused on higher concentration samples. The resuhs indicate that ecological risks of coking wastewater still exists, though it has been treated by conventional progress, and we should also take necessary toxic tests such as its phytotoxicity to plants, to provide further evidence for assessing the security of coking wastewater to environment.