中文摘要：

目的：检测新疆哈萨克族食管癌和癌旁组织中PLCEI基因表达水平，分析PLCEl基因表达与食管鳞癌的分化、分期及转移的相关性。方法：选取30例食管鳞癌及30例配对癌旁组织，应用实时荧光定量PCR（SYBRGreenReal-timePCR）检测PLCEl基因的mRNA表达，分析其与食管鳞癌临床病理特征的关系。结果：PLCElmRNA在食管鳞癌的表达水平明显高于在癌旁正常组织的表达，差异具有统计学意义（P=0．012）；PLCElmRNA的表达与肿瘤分期、淋巴结转移相关，差异具有统计学意义（P〈0．05）；与肿瘤部位、肿瘤大小及分化程度无关，差异无统计学意义（P〉0．05）。结论：PLCEI与食管鳞癌的分期、淋巴转移有关，而与食管鳞癌的分化程度无明显关系。提示PLCEl的高表达在食管鳞癌的发生、浸润及转移过程中可能具有重要作用。

英文摘要：

Objective： To investigate the expression of PLCEI gene mRNA in Kazakhg esophagas squamous cell carci- noma （ESCC） and explore the relationship of PLCE1 expression to the differentiation degree ,stage ,invasion and metastasis of ESCC. Methods：real-time fluorescent quantitative PCR assay was employed to detect the expression of PLCE1 mRNA in 30 paraffin-embedded Xinjiang Kazakh ESCC and 30 non-cancerous adjacent tissue（NCAT）. Restflts：The expression of PLCEI mRNA in ESCC was significant higher than that in normal-appearing mueosa（P =0. 022）;the expression of PLCE1 mRNA was significantly associated with regional lymph node metastasis （ P = 0. 002 ） and pathological staging（ P = 0.022）, the expres- sion of PLCE1 mRNA had no relationship with the degree of differentiationgender ,age,tumor location and tumor size （P 〉 0. 05）. Conclusion：The over-expression of PLCEI mRNA was related to the genesis or development or metastasis of ESCC and might be a useful parameter as an indication of tumor invasion, metastasis, as well as a noval target site for gene therpy.