中文摘要：

目的：研究麦冬皂苷D对HUVEC凋亡相关分子的影响，探讨其作用机制。方法：用H20：构建凋亡模型，运用MTT及流式细胞仪检测细胞活性，激光共聚焦方法测定粒体膜电位和钙离子浓度。结果：麦冬皂苷D可以稳定线粒体膜电位。减少钙离子内流，增加细胞的活力。结论：麦冬皂苷D对HUVEC有一定的保护作用。

英文摘要：

Objectives： To investigate the effect of ophiopogonin D on the apoptosis of vascular cell inducted by H2O2, and to reveal the molecular mechanism, Method： Apoptosis model of HUVEC was established by inducing with H2O2. The activity of HUVEC cell was detected by MTT assay with flow cytometry. Mitochondrial membrane potential and Ca^2＋ concentration in HUVEC was determined by laser scanning confocal microscope. Results： The presence of ophiopogonin D increased the activity of HUVEC cell by stabilizing mitochondrial membrane potential and decreasing intracellular Ca^2＋. Conclusions： Ophiopogonin D protects the vascular endothelial cell against apoptosis induced by H2O2.