中文摘要：

目的探讨正常鼠骨髓间充质干细胞（BM—MSCs）移植对MRI/lpr狼疮鼠B细胞活化因子（BAFF）表达及B细胞活化的影响。方法18只雌性MRL／lpr鼠随机分为MSCs治疗组和对照组，18周龄时治疗组经尾静脉移植MSCs 1×l0^6／只；5只同周龄雌性BALB／C小鼠作为健康阴性对照。酶联免疫吸附试验（EuSA）法检测血清BAFF、干扰素（IFN）-γ、向细胞介素（IL）-2、IL-10水平，流式细胞术检测脾脏中边缘区、T1期、T2期B细胞百分率及细胞数。结果①MSCs治疗8周后，治疗组血清BAFF水平[（32±14）ng／ml]显著低于对照组[（47±13）ng／ml]（P〈0．05）；血清IL-10[（19±7）pg／ml]显著低于对照组[（40±13）pg／ml]（P〈0．01）；血清IFN-γ、IL-2低于对照组[（26±20）pg／ml与（38±25）pg／ml、（73±10）pg／ml与（80±14）pg／ml]，但差异无统计学意义。②MSCs治疗8周后，可降低治疗组脾脏边缘区B细胞百分率（15±4）％，对照组为（21±5）％，但差异无统计学意义，并能显著降低边缘区B细胞数[（9±6）×10s与（19±10）x106；p〈0．0530③MSCs治疗8周后，可降低治疗组脾脏T1期、T2期B细胞百分率[（3．4±2．1）％与（7．3±4．0）％]、[（2．6＋1．4）％与（4．8-＋2．7）％]，但差异无统计学意义，并能显著降低T1期B细胞绝对数[（2．7±1．7）×10^6与（5．1±2．0）×10^6 P〈0．05]、T2期B细胞绝对数[（2．0±1．2）×10^6与（3．7±1．7）×10^6,P〈0．0530结论BM—MSCs移植可能通过抑制体内BAFF的过量表达，进而抑制狼疮鼠B细胞的过度活化。

英文摘要：

Objective To investigate the effect and mechanism of murine bone marrow mesenchymal stem cells （BM-MSCs） transplantation on B-cell activating factor （BAFF） expression and B cell activation of MRL/lpr mice. Methods Eighteen female MRL/lpr mice were divided into the treatment group and the control group. Five female BAL B/C mice were used as negative controls. At the age of 18 weeks, the treatment group was transplanted with 1：：〈106 murine BM-MSCs through vena caudalis, the control group was treated with 0.5ml sodium chloride. Enzyme linked immunosorbent assay （ELISA） was used to measure the level of the BAFF, IFN-γ, IL-2 and IL-10 in the serum. The percentage and numbers of Marginal zone, T1 and T2 B cells in spleen were detected by flow cytometry. Results ① Eight weeks after transplantation, the level of BAFF [（32±14） ng/ml] in serum of the treatment group decreased significantly than the control group [（47±13） ng/ml] （P〈0.05） as well as the level of serum IL-10, IFN-γ and 1L-2 levels [（19±7） vs （40±13） pg/ml] （P〈0.01） [（25±20） pg/ml vs （38±25） pg/ml] [（73±10） pg/ml vs （80±15） pg/ml]. ② Eight weeks after trans-plantation, the mice in the treatment group had lower percentages of marginal zone B cells [ （ 15±4）% vs （21±5）%], and the numbers of marginal zone B cells were significantly decreased in the treatment group as compared with the control group [（9±6）×10^6 vs （19±10）×10^6, P〈0.051. ① Eight weeks after transplantation, the mice in the treatment group had lower percentages of TI and T2 B cells[（3.4±2.1 ）% vs （7.3±4.0）% ][（2.6±1.4）% vs （4.8±2.7）%], and the numbers of T1 [（2.7±1.7）×10^6 vs （5.1±2.0）×10^6, P〈0.05] and T2 B cells [ （ 2.0±1.2 ）×10^6 vs （ 3.7±1.7 ）×10^6, P〈0.05 ] were both significantly decreased in the treatment group as compared with the control group. Conclusion BM-MSCs transplantation decreases the expression of BAFF in association with the diminish