中文摘要：

目的探讨系统性红斑狼疮（SLE）患者骨髓间充质干细胞（MSCs）多向分化能力是否存在异常。方法密度梯度离心法和贴壁筛选法分离培养MSCs，定向诱导骨髓MSCs向脂肪和成骨细胞分化，脂肪细胞经油红O染色鉴定并定量，成骨细胞经茜素红S染色鉴定。将骨髓MSCs与羟基磷灰石共孵育，将共孵育物植于裸鼠皮下，8周后常规苏木素-伊红（HE）染色。反转录-聚合酶链反应（RT—PCR）检测过氧化物酶体增殖物激活受体γ-2（PPARγ-2）、脂蛋白酶（LPL）、Runx2／CBFA1、降钙素（osteocalcin）mRNA的表达水平。结果SLE骨髓MSCs分化成脂肪细胞比例低于健康对照组，油红O染色定量低于健康对照组[（35±7）％与（80±5）％]，（0．14±0．04与0．27±0．04），LPLmRNA表达低于健康对照组（0．369±0．020与0．481±0．038），两组PPARγ-2mRNA表达差异无统计学意义（0．421±0．052与0．441±0．012）。SLE骨髓MSCs分化成骨细胞后形成钙结节低于健康对照组[（35±4）％与（45±4）％]，Runx2／CBFA1、osteocalcin mRNA表达低于健康对照组（0．371±0．000与0．563±0．069），（O．819±0．023与0．962±0．049）；羟基磷灰石与SLE患者骨髓MSCs共孵育后移植裸鼠皮下8周后，成骨细胞形成明显少于健康对照组。结论SLE骨髓MSCs成脂和成骨分化能力存在异常，提示SLE患者骨髓MSCs存在缺陷。

英文摘要：

Objective To-investigate themuhilineage differentiation potential of bone marrow-derived mesenchymal stem cells （MSCs） in patients with systemic lupus erythematosus （SLE）. Methods Density gradient centrifugation and plastic adherence methods were used for isolation of marrow-derived MSCs. Then their differentiation potentiality to lipoblasts and osteoblasts was tested. MSCs loading on hydroxyapatite were embedded in the nude mouse＇s subcutaneous tissues. Eight weeks later, osteogenesis was evaluated by HE staining. PPARγ-2, LPL, Runx2/CBFA1, osteocalcin gene expression in MSCs after differentiation were examined by RT-PCR. Results The positive rates of lipoblasts stained by oil red O and optical density in SLE were decreased than in the control group [（35±7）% vs （80±5）%] （0.14±0.04 vs 0.27±0.04）, and the positive rates of osteoblasts stained by Alizarin Red S in SLE were decreased than those in the control group [ （35±4）% vs （45±4）% ]. Osteoblast differentiation in the SLE group was less than that of the control group. The mRNA expression of LPL （0.369±0.020 vs 0.481±0.038）, Runx2/CBFA1 （0.371±0.000 vs 0.563±0.069）, osteocalcin （0.819±0.023 vs 0.962±0.049） of MSCs after differentiation in the SLE group was decreased than that of the control group. There was no significant difference in the expression of PPARγ-2 mRNA between SLE and control group （0.421±0.052 vs 0.441±0.012）. Conclusion MSCs from SLE have abnormalities in osteogenic and adipngenic differentiation potential.