中文摘要：

为验证水的浊度、pH值及温度等因素对藻红蛋白（PE）含量检测的影响,以浓度范围5~180mg/L PE配置的标准液为研究对象,采集不同浓度标准液的可见光吸收光谱,运用偏最小二乘法（PLS）建立不同谱区的PE定量分析模型。实验结果表明,480~570nm谱区所建模型相关性最高,相关系数R2可达0.993 0,预测结果误差最小;同时,对0~90NTU不同浊度的PE标准液进行浊度补偿分析,建立了不同分析模型下的浊度补偿函数;分析了温度9.7~52.8℃,pH在4.2~10.5范围内PE的光谱特性,当温度在40℃以下时PE的光谱特性基本不发生变化,而pH在6.0~9.0变化时影响可以忽略。本文研究可为海水中PE含量的在线检测参考。

英文摘要：

Phycoerythrin （PE） is an important indicator which measures the algae eutrophication in water. In order to verify the influence of turbidity, pH and temperature on determination of PE,the visible absorption spectra of with concentration the standard solutions of PE in the range of 5-180 mg/L are collected. The partial least squares （PLS） algorithm is used to establish the quantitative analysis models for different spectral regions. The experimental results show that the model in the spectral region of 480-570 nm have the highest correlation and the minimum error. By analyzing the phycoerythrin solutions different turbidity of 0-90 NTU, the turbidity compensation function for the different spectral region is established by analysis model. By analyzing the PE spectral characteristics in the temperature range of 9.7-52.8℃ ,pH of 4. 2-10. 5 ,the PE spectral characteristics almost do not change when the temperature is below 40℃ and the pH is in the range 6.0-9.0. It can lay the foundation for on-line detection of PE content in seawater.