中文摘要：

目的宫颈癌是最常见的妇科恶性肿瘤之一,本研究拟在血清学和组织学水平共同探讨宫颈癌患者蛋白磷酸酯酶2A（PP2A）水平的变化。方法分别选择同期宫颈无病变健康体检者（健康对照组）30例,高危型人乳头瘤病毒（hsHPV）感染患者17例和宫颈癌患者23例,采用酶联免疫吸附试验检测血清PP2A蛋白表达水平;另选取健康对照者24例,hsHPV感染患者18例和宫颈癌患者14例,采用酶活试剂盒检测组织PP2A活性。结果与健康对照组相比,hsHPV感染组血清PP2A水平下降,差异有统计学意义（P〈0.05）,同时组织PP2A酶活性下调,PP2A活性仅为健康对照组的66%,差异有统计学意义（P〈0.05）;宫颈癌组血清PP2A水平下降明显,差异有统计学意义（P〈0.05）,同时组织酶活性显著下调,PP2A酶活性仅为健康对照组的45%,差异有统计学意义（P〈0.05）。与hsHPV感染组相比,宫颈癌组患者血清PP2A水平下调差异有统计学意义（P〈0.05）,组织酶活性水平下调差异也有统计学意义（P〈0.05）。结论宫颈癌患者血清PP2A蛋白表达水平显著下调,同时组织学水平PP2A酶活性亦下降。该研究结果为宫颈癌发病机制提供了新的资料,为宫颈癌的早期诊断提供了新的靶标分子。

英文摘要：

Objective Cervical cancer was one of the most common gynecologic malignancy,this study aimed to discuss serum protein phosphatase 2A（PP2A）levels in patients with cervical cancer at the serological and histological levels.Methods 30 cases of healthy people without cervical lesions（normal control group）,17 cases of patients infected by hsHPV（hsHPV group）,and 23 cases of patients with cervical cancer（cervical cancer group）were selected.The serum PP2 Alevels of three groups were detected by ELISA,and the tissue protein PP2 Alevels were detected by enzyme activity assay.Results Comparing with normal control group,the serum level of PP2 Ain hsHPV group significantly decreased（P〈0.05）,and the PP2 Aactivity in tissues also decreased in the hsHPV group（P〈0.05）,accounting for only 66% of normal control group.The serum PP2 Alevel in cervical cancer group was significantly lower than normal control group（P〈0.05）,and as well as the PP2 Aactivity in tissues（P〈0.05）,accounting for only 45% of normal control group.Comparing with hsHPV group,cervical cancer group had significantly lower serum PP2 Aand tissue PP2Aactivity（P〈0.05）.Conclusion Both of the serum PP2 Alevel and its activity in tissues significantly decreased in patients with cervical cancer.The result of this study could provide new data for the mechanism research of cervical cancer and a new target molecule for its early diagnosis.