中文摘要：

目的：研究半夏、夏枯草药对配伍后对夏枯草中迷迭香酸提取率的影响。方法：采用Thermo Scientific-C18色谱柱（250 mm×4.6 mm,5μm）,以甲醇-1%甲酸（30∶70）为流动相,流速为1 m L/min,检测波长为330 nm,柱温40℃。结果：迷迭香酸进样量在0.1-0.5μg范围内有良好的线性关系（r=0.9998）,平均回收率为101.1%（RSD=1.71%,n=6）。双夏汤配伍后,半夏与夏枯草比例大于1.5∶1时迷迭香酸提取率降低,小于1.5∶1时迷迭香酸提取率升高。结论：双夏汤不同配伍中半夏的含量影响夏枯草中迷迭香酸的提取率。

英文摘要：

Objective： To study the effect of pinellia combined with prunellae vulgaris in different ratios on the extraction yield of rosmarinic acid. Methods： A Thermo Scientific-C18 column（ 250 mm × 4. 6 mm,5 μm） was used. The mobile phase was the mixture of methanol-1% formic acid（ 30∶ 70）. The flow rate was 1 m L / min and the UV detection wavelength was 330 nm. The column temperature was 40 ℃. Results： The linear range of rosmarinic acid was 0. 1 μg-0. 5 μg（ r = 0. 9998） and the average recovery rate of rosmarinic acid was 101. 1%（ RSD = 1. 71%,n = 6）. Compared with prunellae,the extraction yield of rosmarinic acid was reduced when pinellia and prunellae ratio was greater than 1. 5 ∶ 1. And the extraction yield of rosmarinic acid was increased when pinellia and prunellae ratio was less than 1. 5∶ 1. Conclusion： Pinellia influents the extraction rate of rosmarinic acid in Shuangxia Decoction.