中文摘要：

目的：研究水蛭提取液对人视网膜色素上皮（retinal pigment epichelial，RPE）细胞PAR-1表达的影响。方法：水蛭提取液体外单独或与凝血酶共同作用于人RPE细胞后，用免疫荧光法测定PAR-1的荧光表达。结果：PAR-1的免疫荧光光密度值比较：（1）凝血酶组与正常对照组比较，PAR-1的IOD值下降（1608±675，4036±1134，P〈0．01）。（2）单加水蛭提取液组与正常对照组比较，PAR-1的IOD值升高（9998±2374，4036±1134，P〈0．01）。（3）凝血酶和水蛭提取液同时加药组与凝血酶组比较，PAR-1的IOD值显著升高（19664±5436，1608±675，P〈0．01）。表明水蛭提取液能竞争性抑制凝血酶对PAR-1的活化作用。结论：水蛭提取液能抑制凝血酶诱导的人RPE细胞膜上的PAR-1的活化，阻断PAR-1介导的细胞信号传导。

英文摘要：

AIM： To research the influences of leech extracting solution on expression of thrombin receptor-1 in cultured human retinal pigment epithelial（RPE） cells. METHODS： After RPE cells were treated by leech extracting solution alone or leech extracting solution with thrombin, flourescence expression of PAR-1 was mea sured by immumofluorescence method. RESULTS： Flourescence integral optical density（ IOD） of PAR-1 displayed that thrombin group： compared with normal control group, IOD decreased （P 〈 0.01 ） ; leech extract group： compared with normal control group, IOD increased （P 〈 0. 01 ）; leech extracting solution with thrombin group： compared with thrombin group, IOD significantly increased （ P〈 0.01 ）. CONCLUSION：Leech extracting solution seems to be a competitive inhibitor of thrombin during PAR-1 activation in hRPE cells. It can inhibit the activation of PAR-1 by thrombin in human RPE cell membrane and PAR-l-mediated cell signal transduction.