中文摘要：

目的：研究胚胎干细胞相关基因Oct4和Nanog在人胰腺癌肿瘤干细胞和普通肿瘤细胞中表达的差异。方法：用流式细胞仪在人胰腺癌Panc-1细胞系中分选肿瘤干细胞，细胞免疫荧光法检测2种肿瘤干细胞和Panc-1细胞中Oct4、Nanog基因的表达情况。结果：Oct4及Nanog基因在2种分选出的细胞中表达均高于未分选细胞。结论：干细胞相关基因Oct4、Nanog在胰腺癌Panc-1肿瘤干细胞中的表达高于普通胰腺癌细胞。

英文摘要：

Objective：To study and compare the gene expresse of Oct4 and Nanog in human pancreatic cancer stem cell. Methods： FCM was used to isolate pancreatic cancer stem cells of CD44＋CD24＋ and CD44＋CD24＋ESA＋ phenotypes by a FACS Aria II in human cell line Panc-1. Immunofluorescence was used to detect the expression of Oct4 and Nanog gene in Panc-1 cell and two kinds of cancer stem cell-like cells. Results：Oct4 and Nanog genes have a higher expression in CD44＋CD24＋ESA＋ cells and CD44＋CD24＋ cells than Panc-1 cells. Conclusion：Oct4 and Nanog gene perform a higher expression in pancreatic cancer stem cells than in Panc-1 cells.