中文摘要：

目的：探讨Let-7f对胰腺癌细胞侵袭和迁移能力的影响。方法：采用RT-q PCR观察PANC-1、Bx-PC3与正常胰腺细胞HPDE6C7中Let-7f和MMP-11的表达差异;采用Let-7f模拟物和抑制物调节胰腺癌细胞中Let-7f的表达,利用Transwell小室分析Let-7f表达对胰腺癌细胞侵袭和迁移能力的影响;采用RT-q PCR观察干预Let-7f对MMP-11表达的影响。结果：RT-q PCR显示胰腺癌细胞中Let-7f水平明显低于HPDE6C7细胞（P〈0.05）,MMP-11水平明显高于HPDE6C7细胞。上调胰腺癌细胞中Let-7f的表达,可明显抑制其侵袭和迁移（P〈0.05）,下调Let-7f的表达,无显著变化。Let-7f抑制MMP-11表达（P〈0.05）。结论：Let-7f可能通过调控MMP-11的表达影响胰腺癌的侵袭和迁移,为胰腺癌生物治疗提供新靶点。

英文摘要：

Objective To investigate the influence of Let-7f regulation on MMP-11 in migration and invasion of pancreatic cancer. Methods RT-q PCR was performed to assay the expression of Let7 and MMP-11 expression in pancreatic cancer cells and normal pancreatic cells. Let-7f mimic and inhibitor were transfected to PANC-1 and Bx-PC3, respectively. The influence of Let-7f expression on migration and invasion of pancreatic cancer cells was analyzed by transwell. The influence of Let-7f intervention on MMP-11 expression was observed by RT-q PCR. Results Let-7f expression decreased in PANC-1 and Bx-PC3, compared to HPDE6c7, while MMP-11 expression up-regulated in PANC-1 and Bx-PC3, compared to HPDE6c7 cells. Let-7f overexpression inhibited the expression of MMP-11. Up-regulated Let-7f could significantly inhibit migration and invasion of PANC-1 and Bx-PC3 and Let-7f inhibitor enhanced MMP-11 expression. However, down-expression of Let-7f could not significantly promote migration and invasion abilities of PANC-1 and Bx-PC3 cells. Conclusions Let-7f may affect migration and invasion by regulating MMP-11 expression in pancreatic cancer, suggesting that Let-7f might be a potential target for gene therapy in human pancreatic cancer.