中文摘要：

为了提供葡萄转抗病基因功能验证及遗传转化研究的再生技术体系,以中国野生华东葡萄（V.pseudoretic-ulata）感白粉病株系广西-2的花药为试材,进行了体细胞胚诱导与再生体系的建立,并探讨了次生胚的诱导与畸形萌发胚状体正常成苗的方法。结果表明,广西-2花药在B5＋6-BA4.0mg·L^-1＋2,4-D0.7mg·L^-1＋CH1.0g·L^-1＋PVP1.0g·L^-1＋蔗糖30.0g·L^-1培养基上继代3个月,可诱导胚性愈伤组织产生;B5＋6-BA4.0mg·L^-1＋CH1.0g·L^-1＋PVP1.0g·L^-1＋蔗糖15.0g·L^-1培养基有利于胚状体形成;胚状体在1/2B5＋CH1.0g·L^-1＋PVP1.0g·L^-1＋蔗糖15.0g·L^-1的液体培养基比相同成分固体培养基中早萌发14d左右;已萌发胚状体在WPM＋IBA0.15mg·L-1＋AC0.5g·L^-1＋蔗糖15.0g·L^-1培养基上可以生根成苗;MS＋6-BA1.5mg·L^-1＋IBA0.2mg·L^-1＋蔗糖30.0g·L^-1培养基有利于畸形萌发胚状体正常成苗。发育早期胚状体在B5＋6-BA1.0mg·L^-1＋2,4-D0.5mg·L^-1＋CH1.0g·L^-1＋PVP1.0g·L^-1＋蔗糖30.0g·L^-1培养基上可形成次生胚。

英文摘要：

In order to offer a regeneration system for functional characterization of resistant genes in grapevine genetic trans-formation,anthers from powdery mildew-susceptible line Guangxi-2 of Chinese wild Vitis pseudoreticulata were used to induce somatic embryos and establish regeneration system. The induction of secondary embryos and the normal seedling from abnormal germinated somatic embryo were also explored in this study. The results indicated Guangxi-2 anthers could be induced and formed embryo callus on B5 medium containing 6-BA 4.0 mg·L^-1＋ 2,4-D 0.7 mg·L^-1＋ CH 1.0 g·L^-1＋ PVP 1.0 g· L^-1＋sucrose 30.0 g·L^-1 after 3-month subculture. The B5 medium containing 2,4-D 0.7 mg·L^-1＋ CH 1.0 g·L^-1＋ PVP 1.0 g·L^-1＋ sucrose 15.0 g·L^-1 was beneficial to form embryos. The germination of embryos on the1/2 B5 liquid medium containing CH1.0 g·L^-1＋PVP 1.0 g·L^-1＋sucrose 15.0 g·L^-1 was 14 days earlier than on the B5 solid medium. The medium for root formation on the germinated embryo was WPM medium containing IBA 0.15 mg·L^-1＋ AC 0.5 g·L^-1＋ sucrose 30.0 g·L^-1. The MS medium containing 6-BA 1.5 mg·L^-1＋ IBA 0.2 mg·L^-1＋ sucrose 30.0 g·L^-1 was favorable to the normal seedling formation from mal-formed embryos. The embryos in early developmental stage could be generated secondary somatic embryos on the B5 medium containing 6-BA 1.0 mg·L^-1＋2,4-D 0.5 mg·L^-1＋ CH 1.0 g·L^-1＋ PVP 1.0 g·L^-1＋ sucrose 30.0 g·L^-1.