中文摘要：

以中国原产抗霜霉病葡萄野生种华东葡萄（Vitis pseudoreticulata）白河-35-1为材料，用SMART^TM技术构建葡萄霜霉菌（Plasmopara viticola）诱导的葡萄叶片cDNA文库。经检测，原始文库滴度0．92×10^6pfu／mL，重组率97％，插入片段500～2200bp，扩增文库滴度为5×10^9pfu／mL。随机挑取克隆5’端测序，获得254条有效EST序列（GenBank登录号：FG269201-FG269449和FG396006-FG396010o经BlastX分析，有56．3％ESTs与GenBank中功能已知基因有较高同源性，14．6％ESTs与功能尚未确定的假设蛋白或未知蛋白同源性很高，20．9％ESTs在GenBank中无匹配的同源产物。

英文摘要：

A cDNA library was constructed with Vitis pscudoreticulata clone Baihe-35-1 leaves inoculated by Plasmopara viticola using SMART^TM cDNA Library Construction Kit. The titer of primary library and amplified library was about 0.92× 10^6 pfu/mL and 5× 10^9 pfu/mL, respectively. In the primary library, 97% clones were recombinant and the length of insert cDNAs were from 500 to 2 200 bp. Two hundred and fifty-four ESTs （GenBank accession No. FG269201-FG269449 and FG396006-FG396010） were obtained by random sequencing of the cDNA library. BlastX search showed that 56.3% ESTs had higher homology with functional genes, 14.6% ESTs with hypothetical putative or unknown protein, and 20.9% ESTs had no-hits.