中文摘要：

目的：建立UPLC法同时测定葛根芩连汤肠外翻囊样品中6个黄酮类成分（葛根素、大豆苷、甘草苷、野黄芩苷、黄芩苷、汉黄芩苷）的含量。方法：样品用甲醇提取，采用Agilent Proshell 120EC—C18（46mm×50mm，2．7μm）色谱柱，以0.2％醋酸水溶液（A）-甲醇（B）为流动相进行梯度洗脱，流速1mL·min^-1，紫外检测波长270nm，柱温30℃。结果：葛根素、大豆苷、甘草苷、野黄芩苷、黄芩苷、汉黄芩苷质量浓度分别在0．1870—93．50μg·mL^-1（r=n9999），0.2730-13．65μg·mL^-1（r=0.9999），0.02550～12．75μg·mL^-1（r=0．9999），0．05580～27．90μg·mL^-1（r=0.9998），0.2124—106．2μg·mL^-1（r=0．9999），0.09140—45．70μg·mL^-1。（r=0．9999）范围内有良好的线性关系；日间、日内精密度变异均小于2％；平均回收率（n=9）分别为101．4％，97．88％，99．15％，99．72％，98．50％，101．4％；稳定性良好。结论：所建立的分析方法操作简便、快速、灵敏，结果准确，重复性好，所需样品少，并已成功应用于葛根芩连汤大鼠肠吸收动力学的研究。

英文摘要：

Objective ：To develop a sensitive and selective ultra performance liquid chromatography （UPLC） method for identification and quantification of six flavonoids components （ puerarin, daidzin, liquiritin, seutellarin, baicalin, wogonoside） in rat everted gut sacs. Methods ：The method was based on simple liquid -liquid extraction. The separation was performed on a Agilent Proshell 120 EC - C18 （4. 6 mm× 50 mm, 2. 7 μm） column. The mobile consisted of 0. 2% acetic acid aqueous（A） and methanol（B） with a gradient elution at a flow-rate of 1.0 mL · min^-1. The detection wavelength was 270 mn and the column temperature was 30 ℃. Results：The linear ranges of puerarin, daidzin, liquiritin, scutellarin, baiealin, wogonoside were 0. 1870 -93. 50 μg mL^-1 （r =0. 9999） ,0. 02730 - 13. 65 μg· mL^-1 （r =0.9999）,0.02550-12.75 μg· mL^-1（r=0.9999）,0. 05580 - 27. 90 μg· mL^-1 （r =0.9998）,0. 2124-106.2 μg· mL^-1 （r =0. 9999） ,0. 09140 -45. 70 μg· mL^-1 （r =0. 9999）, respectively. The intra - and inter -day precisions（RSD） were within 2% for the analytes. The mean recoveries of puerarin, daidzin, liquiritin, scutellarin, baicalin, wogonoside were 101.4%, 97. 88%, 99. 15%, 99. 72%, 98. 50%, 101.4%. Conclusion：The proposed method enables unambiguous identification and quantification for the preliminary pharmacokinetic studies of the compounds （ puerarin, daidzin, liquiritin, scutellarin, baicalin, wogonoside）, and has been applied to a intestinal absorption kinetics of Gegenqinlian decoction in rats successfully.