中文摘要：

目的利用复合松质骨基质（Cancellous bone matrix,CBM）的中空多孔钛假体（Hollow porous titanium prostheses,HPTP）观察兔体内假体周围及内部骨生成情况,探讨人工假体与骨质整合的改进方法。方法设计中空多孔（孔径2 mm）和无孔两种假体,表面行羟基磷灰石（Hydroxyapatite,HA）喷涂。实验组分为无孔假体组（A组）、HPTP组（B组）和HPTP＋CBM组（C组）,每组16例。将假体分别植入48只4-6个月龄新西兰大白兔右侧股骨外侧髁,饲养至第3、8、12周时取材,以X线、显微镜、电镜及形态计量软件观察复合假体表面及内部骨生成情况。采用SPSS13.0进行t检验分析。结果三组植入物外表面HA涂层的组织生长情况类似;A组无孔,未见骨长入,B组和C组中骨组织最终可长入假体2 mm大的圆孔;各时间点C组中空腔内骨生长率均明显高于B组（P〈0.01）。结论骨质可经HPTP假体孔洞长入腔内,达到交锁固定,较无孔假体更稳定;中空腔复合CBM后可明显加快骨长入并诱导骨生成,使假体与骨质更好整合。

英文摘要：

Objective To observe the possibility of bone formation in vivo of rabbits with hollow porous titanium prostheses （HPTP） loading cancellous bone matrix （CBM）, so as to improve the osteointegration between prostheses and bone. Methods HPTP （2 mm in diameter） and non-porous prostheses coated with HA were designed. Experimental samples were divided into non-porous prosthesis group （group A）, HPTP group （group B） and HPTP plus CBM group （group C）, with 16 samples in each group. All the implants were implanted in the right lateral femoral condyles of 48 New Zealand rabbits of 4-6 months old, which were fed to 3, 8, 12 weeks later and sacrificed. SPSS13.0 software was used for t-test analysis. Results The tissues around HA-coated implants in the three groups were nearly the same at different time points. No bone grew into Group A because of non-porous design, and bone could grow into the 2 mm round holes both in group B and C ultimately. Bone formation rates in group C were significantly higher than that in group B at different time points （P〈0.01）. Conclusion Bone could grow into the cavity of HPTP prostheses via the round holes to achieve locking fixation effect, which are more stable than non-porous prostheses. CBM could speed up bone ingrowth significantly and induce bone formation to achieve better integration of prostheses and bone.