中文摘要：

分析与FecB（Fec=fecundity,B=Booroola）基因紧密连锁的微卫星座位LSCV043在高繁殖力绵羊（Ovis aries）品种（小尾寒羊）和低繁殖力绵羊品种（特克塞尔和多赛特）中的遗传多态性,同时分析了该微卫星座位与小尾寒羊FecB基因的连锁不平衡关系。高繁殖力的小尾寒羊在骨形态发生蛋白受体IB（bone morphogenetic protein receptor IB,BMPR-IB）基因编码序列第746位碱基处发生了与Booroola Merino绵羊相同的FecB突变（A746G）,而低繁殖力的特克塞尔和多赛特绵羊则没有发生这种突变;小尾寒羊BB、B＋和＋＋基因型频率分别为0.487、0.398和0.115。微卫星座位LSCV043在3个绵羊品种365个个体中共检测到6个等位基因和13种基因型,最小等位基因为98bp,最大等位基因为132bp;小尾寒羊（n=269）、特克塞尔（n=48）、多赛特（n=48）和BB基因型（n=131）、B＋基因型（n=107）、＋＋基因型（n=31）小尾寒羊中频率最大的等位基因分别是132、112、110、110、132和110bp或112bp,多态信息含量分别是0．750、0．769、0．757、0．712、0．762和0．774。连锁不平衡分析显示，小尾寒羊FecB基因B等位基因与LSCV043微卫星座位98bp等位基因之间存在一定的连锁不平衡（D＇=0．464），＋等位基因与LSCV043微卫星座位104bp等位基因存在较强的连锁不平衡（D＇=0．636）。研究结果初步表明，LSCV043微卫星座位98bp等位基因与小尾寒羊FecB基因B等位基因之间存在一定的连锁不平衡关系，是与小尾寒羊多羔主效基因紧密连锁的一个遗传标记。

英文摘要：

Genetic polymorphisms of microsatellite locus LSCV043, which was closely linked to the ovine hyperprolificacy major gene FecB （Fee=fecundity, B=Booroola）, were detected in high fecundity sheep （Ovis aries） breed （Small Tail Han sheep） and low fecundity sheep breeds （Texel and Dorset）. And the linkage disequilibrium between microsatellite locus LSCV043 and FecB gene of Small Tail Han sheep was analyzed. Result showed that there was the same FecB mutation （A746G） of bone morphogenetic protein receptor IB （BMPR-IB） gene in Small Tail Han sheep as that in Booroola Merino ewes. This mutation did not exist in both Texel and Dorset sheep. The frequency of BB, B＋ and ＋＋ genotypes was 0.487, 0.398 and 0.115 in Small Tail Han sheep. Six alleles and 13 genotypes were detected at LSCV043 in 365 individuals from three sheep breeds, in which 98 bp was the shortest allele and 132 bp was the longest allele. The allele of the highest frequency was 132, 112, 110, 110, 132 and 110 bp or 112 bp, the polymorphism information content （PIC） was 0.750, 0.769, 0.757, 0.712, 0.762 and 0.774 in Small Tail Han （n =269）, Texel （n =48）, Dorset （n =48）, BB group （n =131） from Small Tail Han, B＋ group （n = 107） from Small Tail Han,＋＋ group （n =31） from Small Tail Han, respectively. In Small Tail Han sheep, linkage analysis showed that there was a certain linkage disequilibrium between 98 bp allele of microsatellite LSCV043 and B allele ofFecB gene （D＇=0.464）, and strong linkage disequilibrium between 104 bp allele of microsatellite LSCV043 and ＋ allele of FecB gene （D＇=0.636）. These results preliminarily indicated that 98 bp allele of microsatellite LSCV043 is a genetic marker closely linked to B allele ofFecB gene in Small Tail Han sheep.