中文摘要：

目的：观察CD99基因转染对霍奇金淋巴瘤L428细胞株表型、细胞周期与凋亡的影响。方法：L428细胞分为3组：转染CD99基因组、转染空质粒组及空白对照组。采用免疫细胞化学方法检测3组细胞CD15、CD30与CD99的表达,并用流式细胞术分别检测细胞周期和细胞凋亡。结果：与其他2组相比,转染CD99基因组L428细胞CD15、CD30表达消失,但可检测到CD99的表达。3组细胞周期分布存在差异,转染CD99基因组细胞阻滞于G2/M期（F=24.85,P〈0.001）。3组细胞凋亡率相比,差异有统计学意义（F=45.30,P〈0.001）,其中转染CD99基因组细胞凋亡率为（12.7±4.6）%,高于转染空质粒组的（4.0±0.9）%和空白对照组的（3.6±1.6）%（P〈0.05）。结论：CD99基因可能在霍奇金淋巴瘤的发生中起重要作用。

英文摘要：

Aim：To observe the effects of CD99 gene transfection on the phenotype,cell cycle distribution and apoptosis of L428 cells.Methods：L428 cells were allocated into 3 groups：cells transfected with CD99 gene,those with empty plasmid,and L428 cells without treatment.Immunocytochemistry was applied to detect CD15,CD30,and CD99 expression.Cell cycle analysis and apoptosis were determined by flow cytometry.Results：Compared with the other 2 groups,L428 cells transfected with CD99 showed no expression of CD15 or CD30,but that of CD99.In addition,cell cycle arrested at G2/M（F=24.85,P0.001）and increase of cellular apoptosis was detected in L428 cells transfected with CD99（F=45.30,P0.001）.Conclusion：CD99 may play an important role in the occurrence of Hodgkin lymphoma.