中文摘要：

为探测PAT酶长期胁迫下小鼠遗传多态性的变化，应用AFLP技术对饲喂转Bar基因抗除草剂稻谷子二代（ F2）小鼠遗传多态性进行了研究．36只体重18～22 g的F2代小鼠分为实验组（ Z2）和对照组（ C2），分别饲喂转Bar基因稻谷Bar68-1和非转基因稻谷D68．每组3个重复，每个重复6只小鼠，雌雄各半．6对引物组合扩增出108个AFLP条带，其中多态性条带占25．9％．在聚类图上Z2组肝脏和小肠样品与C2组肝脏和小肠样品有微小差异，但差异不明显．结果表明，PAT对小鼠肝脏和小肠的DNA多态性没有明显影响．

英文摘要：

In order to study the influence of long-period PAT force on genetic polymorphism of mice （ Mus mus-culus） , the genetic polymorphism of the second filial generation （ F2 ） mice fed with of Bar-transgenic rice were ana-lyzed.Thirty-six Mus musculus in a SPF grade of F2 mice （20 ±2 g）, half male and half female, were randomly di-vided into one experimental group （Group Z2, fed with rice Bar68-1）and one control group （Group C2, fed with rice D68） with three replications per group and six mice per replication .Using 6 pairs of AFLP primers , a total of 108 bands were amplified , of which 28（25.9%） were polymorphic .There was a tiny difference between liver and small intestine samples of Group Z 2 and Group C2 based on dendrogram , but the difference was not obvious .The results in-dicate that the influence of long-period PAT force on genetic polymorphism of mice （Mus musculus） is insignificant.