为了探明狼蛛猎物果蝇体表挥发性化合物正十五烷对狼蛛觅食过程中嗅觉定位的影响,采用Y型嗅觉仪法进行单因子变量试验,研究狼蛛对不同浓度(10%、20%、40%和80%)、距离(5 cm、10 cm、15 cm和20 cm)、温度(17℃、23℃和28℃)条件下对正十五烷的嗅觉反应。结果显示:浓度为20%时狼蛛对正十五烷气味源端的选择指数最高和在该端的停留时间最长,显著高于其他浓度时的选择指数和停留时间( P〈0.05);随着距离的延长,狼蛛在正十五烷气味源端的选择指数和停留时间与距离呈显著负相关关系( P〈0.01);随着温度的上升,狼蛛在正十五烷气味源端的选择指数和停留时间与温度呈显著正相关关系( P〈0.01)。可见,狼蛛对正十五烷气味有一定的嗅觉反应,可感受猎物的挥发性信息素协助定位猎物。
To explore the effect of a volatile compound n-pentadecane of wolf spiders’ prey, fruit flies, on wolf spi-ders’ olfactory orientation during foraging, a series of single factor experiments were conducted by Y-shaped olfactometer method. The wolf spiders’ responses to n-pentadecan in different concentrations (10%, 20%, 40% and 80%), distances (5 cm, 10 cm, 15 cm and 20 cm), temperatures (17 ℃, 23 ℃ and 28 ℃) were studied, then the wolf spiders’ selec-tion index and residence time were counted. The wolf spiders showed the highest selection index and the longest residence time to 20% n-pentadecan. With the extension of distance, the selection index and residence time showed negative correla-tions with n-pentadecan odor source (P<0. 01). Selection index and residence time were positively correlated with tempera-ture as it rose (P<0. 01). In conclusion, wolf spiders are responsive to the smell of n-pentadecane, which help to feel the prey’ s volatile compound so as to locate preys.