中文摘要：

以烟草叶片为材料,观察涂抹生长素IAA与注射Harpinxoo蛋白的相互作用的表型变化,并对烟草叶组织内的氧爆发现象进行了定量测定及显微观察,同时,选取EF-1α作为内参基因,通过半定量PCR检测HR标志基因、防卫基因及生长素类相关基因的表达情况。表型试验结果表明,10-4mol·L-1IAA能够抑制harpinXoo激发的过敏反应,而500μmol·L-1生长素抑制剂三碘苯甲酸（TIBA）能够缓解10-4mol·L-1IAA的抑制作用;氧爆发测定结果与表型一致,IAA同样减少了活性氧（ROS）的积累;半定量PCR结果显示,IAA对HR标志基因hsr203J肪卫基因chia5,PR-1b以及AUX1,ARF8等生长素相关基因均有不同影响。结果表明,IAA可以抑制harpinXoo蛋白引起的HR,也可以调控氧爆发。

英文摘要：

Tobacco leaves were treated with /AA and harpinxoo to observe the interaction between IAA and harpinxoo protein in tobacco leaves, and the oxidative burst in tobacco leaf tissues by quantitative assay and microscopy. The expressions of hypersensitive response marker gene, disease defense genes and auxin related genes were detected by using semi-quantitative PCR. The EF-la gene was used as an internal control because it is highly conserved and constitutively expressed in eukaryotes. Plant assays showed that 10-4 tool ~ L-1 IAA inhibi- ted the hypersensitive response induced by harpinxoo protein in tobacco leaves and that the inhibition was weak- ened when 500 I~mol ~ L-1 TIBA was applied at the same time. The analysis of the oxidative burst in tobacco leaf tissue was in accordance with the results of plant assays that IAA could reduce the accumulation of ROS. The semi-quantitative PCR results showed that IAA had different effects on the expressions of hypersensitive re- sponse marker gene hsr203J, disease defense gene chia5 and PR-1b, auxin related genes AUX1, ARF8 and so on. It is concluded that IAA can inhibit the hypersensitive response induced by harpinxoo protein and oxidative burst in tobacco leaves.