中文摘要：

目的探讨血管紧张素转换酶（ACE）基因多态性对早发冠心病（CHD）血瘀证的影响。方法运用PCR技术对41例早发冠心病（CHD）血瘀证、45例早发冠心病非血瘀证及38名正常对照组的ACE基因型及等位基因频率进行检测,同时检测一氧化氮（NO）和血浆内皮素（ET）含量。结果早发CHD血瘀证DD基因型及D等位基因频率明显高于早发CHD非血瘀证及正常对照组（P〈0.05或P〈0.01）。早发CHD血瘀证患者ET/NO检测值明显高于早发CHD非血瘀证及正常对照组（P〈0.05或P〈0.01）,且以DD型血瘀证患者最高。结论 ACE基因DD基因型及D等位基因与早发CHD血瘀证发病相关,DD基因型是早发CHD血瘀证的独立危险因素,可增加早发CHD的风险,D等位基因是早发CHD血瘀证的易感基因。早发CHD血瘀证ACE基因多态性与ET/NO密切相关。

英文摘要：

Objective To discuss the impact of polymorphism of angiotensin converting enzyme （ACE） gene on premature coro- nary heart disease（CHD） with blood stasis syndrome. Methods The ACE genotype and allele frequencies were detected by the poly- merase chain reaction （PCR） in 41 cases with premature coronary heart disease and blood stasis syndrome, 45 cases without blood stasis syndrome and premature CHD,and 38 cases as normal control group. The content of nitric oxide （NO） and plasma endothelin （ET） were detected. Results DD genotype and D allele frequency in cases with premature CHD and blood stasis syndrome were significantly higher than that in cases with premature CHD and without blood stasis syndrome and healthy controls （P〈0.05 or P〈0.01）. ET/NO in patients with premature CHD and blood stasis syndrome were significantly higber than that in patients with premature CHD and without blood stasis syndrome and normal control group （P〈0.05 or P〈0.01）. The highest was patients with DD blood stasis syndrome. Conclusion Pathogenesis of the ACE gene DD genotype and D allele was associated with premature CHD with blood stasis syndrome. DD genotype was an independent risk factor for premature CHD with blood stasis syndrome,it could increase the risk of premature CHD. The D allele was a susceptibility gene for premature CHD with blood stasis syndrome. ACE gene polymorphism was closely related to the ET/NO in premature CHD with blood stasis syndrome.