中文摘要：

目的 观察丙型肝炎病毒核心蛋白（HCV core）对乙型肝炎病毒（HBV）基因转录和病毒复制的影响。方法 将不同剂量的HCV core表达质粒（pNKF-HCV cole）转染HepG2．2．15细胞。以空载质粒（pNKF）转染HepG2．2．15细胞作为阳性对照，转染HepG2细胞作为阴性对照。以Northem吸印杂交检测HBV mRNA的转录水平，以Southem吸印杂交检测HBV复制中间体DNA的复制水平。结果 20μg pNKF-HCVP core转染HepG2、2．15细胞后，可使细胞中HBV mRNA转录水平及HBV复制中间体DNA复制水平明显降低，抑制率分别为51．9％及36．5％。10μg及15／μg NKF-HCV core较pNKF转染的HepC,2．2．15细胞的HBV mRNA信号与HBV复制中间体DNA检出信号强度无显著差异。结论 HCV核心蛋白达到一定的剂量后能够在一定程度上抑制HBV的转录与复制。

英文摘要：

Objective To investigate the effects of HCV core protein on regulating HBV transcription and replication.Methods Different dosage of HCV core protein expression plasmids, pNKF-HCV core, were transfected into HepG2.2.15 cells. HepG2.2.15 cells transfected with pNKF vector were used as the positive control, HepG2 cells transfected with pNKF vector were used as the negative control.The transcription level of HBV mRNA and the level of HBV DNA replication intermediates were analyzed by Northern and South- em blot hybridization respectively.Results The transcription level of HBV mRNA and the replication level of HBV replication interme- diates DNA in the HepG2.2.15 cells were significantly decreased by 20μg pNKF-HCV core plasmids, and the inhibition ratio were 51.9% and 36.5% respectively. Meanwhile, 10μg and 15μg pNKF-HCV core plasmids did not show obviously effects on the level of HBV specific transcripts,and replication intennediates DNA. Conclusion This study indicated that the HCV core protein may inhabit HBV transcrion and replicalion if given enough dosage.