中文摘要：

以大菱鲆（Scophthalmus maximus）出血症的病原菌——鳗弧菌（Vibrio anguillarum）SMP1为抗原，制备兔抗血清，测定了抗血清的工作浓度和对抗原的特异性，建立了在大菱鲆肝肾组织中检测SMP1的间接ELISA方法。SMP1人工感染大菱鲆，用建立的间接ELISA对大菱鲆肾脏和肝脏进行检测，结果肾脏的阳性检出率为87．7％，肝脏的阳性检出率为90％。该方法的建立有助于快速准确地鉴定由SMP1引起的大菱鲆疾病。

英文摘要：

An indirect Enzyme-Linked Immunosorbent Assay （ELISA） for rapid diagnosis of pathogenic Vibrio anguillarum has been developed. Antiserum specific for the V. anguillarum SMP1 was produced from a New Zealand white rabbit and used in indirect ELISA. The specificity and sensitivity of the antiserum were tested on the liver and kidney tissues of turbot, in which 87.7% out of 30 kidney samples and 90% of liver samples were positive, respectively.