中文摘要：

为探索昆明白小鼠胚胎干细胞建系方法，将受孕4．5天的昆明白小鼠囊胚用免疫手术法去除滋胚层，然后将内细胞团（ICM）接种于胎鼠成纤维细胞饲养层上培养，形成的胚胎干细胞样集落用胰蛋白酶-EDTA消化法传代，培养后进行相差显微镜观察及碱性磷酸酶染色。结果饲养层上生长的ICM细胞呈典型的ES样细胞集落，传至第8代碱性磷酸酶染色呈强阳性。实验表明免疫手术法适用于昆明白小鼠ES细胞建系，获得的细胞集落具有ES细胞的主要生物学性状。

英文摘要：

To investigate the method of establishing embryonic stem （ES） cell line from Kunming species mouse, inner cell masses （ICMs） isolated by immunosurgery from 4.5 d blastocysts of Kunming species mouse were cultured on the fetal mouse fibroblast cell feeder layers. The ES cell-like colones were passaged by trypsin-EDTA digest method to 8th generation. Identified by phasecontrast microscope observation and AKP staining, the obtained ES-like cells proved to be AKP intensively positive and able to form typical ES-like colones. This research confirmed that immunosurgery is suitable for establishing ES cell lines from Kunming species mouse and the cells obtained still maintain the main characteristics of ES cell.