中文摘要：

目的考察非离子表面活性剂维生素E聚乙二醇1000琥珀酸酯（TPGS）对宝藿苷I抑制乳腺癌细胞MCF-7增殖的影响。方法 MTT法测定宝藿苷I对MCF-7的细胞毒性,用荧光显微镜观察宝藿苷I的细胞摄取,并采用HPLC法测定细胞内的宝藿苷I的量。结果在应用TPGS后,宝藿苷I对MCF-7增殖的抑制作用增强,在宝藿苷I低浓度的情况下作用更显著;当宝藿苷I与TPGS质量比分别为1∶1、1∶2、1∶4时,在MCF-7细胞培养2 h的摄取率分别为29.51%、38.12%、40.37%,与仅用宝藿苷I相比分别提高了27.92%、65.24%、74.99%。结论 TPGS能够增加MCF-7对宝藿苷I的细胞摄取,并增强其对MCF-7的细胞毒作用。

英文摘要：

Objective To investigate the effect of D-a-tocopherol polyethylene glycol 1000 succinate （TPGS） on the inhibition of proliferation of breast cancer cells MCF-7 by baohuoside I. Methods The cytotoxicity of baohuoside I to MCF-7 ceils was determined by MTT assay, the cellular uptake of baohuoside I was detected by fluorescence microscopy, and the intracellular baohuoside I was determined by HPLC. Results The effect of baohuoside I on the inhibition of MCF-7 cell proliferation was enhanced in the presence of TPGS, especially on lower concentration. The uptake rates of MCF-7 within 2 h were 29.51%, 38.12%, and 40.37%, when the proportions of baohuosaide I and TPGS were 1：1, 1：2, and 1：4, respectively. The ratios were increased by 27.92%, 65.24%, and 74.99% compared with those using baohuoside I only. Conclusion TPGS can increase the uptake rate ofbaohuoside I in MCF-7 cells and enhance the inhibition of MCF-7 cell proliferation.