中文摘要：

目的：建立RP-HPLC测定枳壳中水合橙皮内酯、马尔敏、川陈皮素、桔皮素和橙皮油素的含量。方法：采用DiamonsilC18（150mm×4.6mm,5μm）色谱柱,流动相为甲醇（A）-水（B）,梯度洗脱（0～7min,51%A;7～9min,51%A→59%A;9～14min,59%A→60%A;14～15min,60%A→64%A;15～25min,64%A;25～29min,64%A→95%A;29～30min,95%A→100%A;30～36min,100%A）,流速1.0mL.min-1,检测波长324nm,柱温30℃。结果：5个成分均能获得基线分离,水合橙皮内酯、马尔敏、川陈皮素、桔皮素和橙皮油素进样量分别在0.0855～2.052,0.0341～0.819,0.0414～0.993,0.0416～0.998,0.0408～0.978μg范围内呈良好的线性关系;平均回收率（n=6）分别为99.11%（RSD=0.59%）,101.1%（RSD=2.6%）,99.52%（RSD=0.85%）,99.59%（RSD=0.95%）,97.44%（RSD=1.6%）。结论：该法简便快速,重现性好,准确可靠,可作为枳壳药材及饮片中水合橙皮内酯、马尔敏、川陈皮素、桔皮素和橙皮油素5个脂溶性成分测定的有效方法。

英文摘要：

Objective：To establish an RP-HPLC method for simultaneous determination of meranzin hydrate,marmin,nobiletin,tangeretin and auraptene in Fructus Aurantii.Methods：Five lipophilic constituents were separated with Diamonsil C18 （150 mm×4.6 mm,5 μm） column with a flow rate of 1.0 mL·min-1 at 30 ℃ and detected at 324 nm. Mobile phase consisted of methanol（A） and water（B） with gradient elution（0-7 min,51%A;7-9 min,51%A→59%A;9-14 min,59%A→60%A;14-15 min,60%A→64%A;15-25 min,64%A;25-29 min,64%A→95%A;29-30 min,95%A→100%A;30-36 min,100%A）.Results：Five liposoluble constituents were well separated.The linear ranges for meranzin hydrate,marmin,nobiletin,tangeretin and auraptene were 0.0855-2.052,0.0341-0.819,0.0414-0.993,0.0416-0.998,0.0408-0.978 μg,respectively;The mean recoveries（n=6） were 99.11%（RSD=0.59%）,101.1%（RSD=2.6%）,99.52%（RSD=0.85%）,99.59%（RSD=0.95%）,97.44%（RSD=1.6%）,respectively.Conclusion：This method is simple,reproducible,accurate,reliable for determination of meranzin hydrate,marmin,nobiletin,tangeretin and auraptene in Fructus Aurantii.