中文摘要：

目的：探讨瘦素（1eptin）在原始卵泡启动生长中的作用及其机制。方法：利用2日龄大鼠离体卵巢体外培养模型，在Waymouth培养体系中分别添加瘦素、瘦素抑制剂（1eptin antagonist）以及ERKl／2信号通路特异性抑制剂（PD98059），通过形态学观察原始卵泡启动生长的变化，Westem blotting检测卵泡ERKl／2、磷酸化．ERKl／2（P—ERKl／2）蛋白表达量的变化。结果：瘦素能够促进原始卵泡的启动生长俨〈O．05），还可激活卵泡ERKl／2信号通路中的ERKl／2蛋白磷酸化（P〈0．05）；用瘦素抑制剂和PD98059可显著抑制瘦素促原始卵泡生长效应（P〈0．05），亦可显著抑制瘦素对卵泡ERKl／2蛋白磷酸化（P〈0．05）。结论：瘦素能够促进原始卵泡的启动生长，其作用机制可能与ERKl／2信号通路有关。

英文摘要：

Objective： To explore the role of leptin in the initiation of rat primordial follicles. Methods： Ovaries of 2-day-old SD rats were cultured for 8 d in the Waymouth culture medium and divided into 6 groups which were supplemented with leptin, leptin antagonist and PD98059 respectively or combinedly. Growth changes of primordial follicles were observed by the morphology, and the expressions of ERK1/2 and P-ERK1/2 in the ovaries cultured in the groups were detected by Western blotting. Result： l） Leptin could accelerate the initiation growth of primordial follicles （P〈0.05）. 2） Leptin could induce the activation of ERK1/2, and the expression of P-ERK1/2 in the ovaries was increased after leptin treatment （P〈0.05）. 3） The growth of primordial follicles activated by leptin via ERK1/2 pathway was blocked by leptin antagonist and PD98059 （P〈0.05）, respectively. Conclusion： Leptin may initiate rat primordial follicles growth via ERK1/2 pathways, suggesting an important role of leptin in rat primordial follicles initiation and development.