本试验旨在研究饲粮中添加25羟基维生素D3(25-OH-D3)和1α羟基维生素D3(1α-OH-D3)代替维生素D3对42~63日龄黄羽肉鸡生长性能、血清生化指标、胫骨发育和养分消化率的影响。试验选用42日龄黄羽肉公鸡2 400只,随机分成8个处理,每个处理5个重复,每个重复60只鸡。处理1(正常钙磷饲粮对照组)的维生素D3、钙、磷含量分别为2 780 IU/kg、0.8%、0.55%;处理2(低钙磷饲粮对照组)~8的维生素D3、钙、磷含量分别为0 IU/kg、0.7%、0.45%;处理3~5中25-OH-D3的添加水平分别为50、70、90μg/kg,处理6~8中1α-OH-D3的添加水平分别为2.5、5.0、10.0μg/kg。试验期21 d。结果表明:1)与低钙磷饲粮对照组相比,除添加50μg/kg 25-OH-D3未使黄羽肉鸡平均日采食量(ADFI)有显著变化(P〉0.05)外,添加不同水平的25-OH-D3或10.0μg/kg 1α-OH-D3均可显著提高黄羽肉鸡生长性能(P〈0.05),添加70、90μg/kg 25-OH-D3或2.5、5.0、10.0μg/kg 1α-OH-D3均可显著提高钙表观消化率和真消化率(P〈0.05),添加5.0、10.0μg/kg 1α-OH-D3可显著提高磷表观消化率和真消化率(P〈0.05);添加50、70、90μg/kg 25-OH-D3或10.0μg/kg 1α-OH-D3均可显著提高血清磷含量和显著降低血清钙磷比(P〈0.05),添加90μg/kg 25-OH-D3或10.0μg/kg 1α-OH-D3还可显著降低血清抗酒石酸酸性磷酸酶(TRAP)活性(P〈0.05),但各处理血清碱性磷酸酶(ALP)活性差异不显著(P〉0.05);添加90μg/kg 25-OH-D3或10.0μg/kg 1α-OH-D3可显著提高胫骨粗灰分重、钙磷比、鲜重、脱脂重、脱脂重指数、长度、折断力和骨密度(P〈0.05),但对胫骨粗灰分率、钙含量和鲜重指数均没有显著影响(P〉0.05)。2)与正常钙磷饲粮对照组相比,添加70、90μg/kg 25-OH-D3或2.5、5.0、10.0μg/kg 1α-OH-D3均可显著提高钙表观消化率(P〈0.05),添加10.0μg/kg 1α-OH-D3可显著提高磷表观消化率和真消化率(P〈0.05?
This study was conducted to investigate the effects of 25-OH-D3 and 1 ct-OH-D3 replacing vitamin D3 on growth performance, serum biochemical indices, tibia development and nutrient digestibility of yellow-feathered broilers aged from 42 to 63 days. A total of 2 400 male yellow-feathered broilers aged 42 days were randomly divided into 8 groups with 5 replicates per group and 60 chickens in each replicate. Treatment 1 (control group ) was fed a basal diet, in which the contents of vitamin D3, calcium, phosphorus were 2 780 IU/kg, 0.8%, and 0. 55%, respectively. For treatments 2 (low phosphorus and calcium control group) to 8, the contents of vitamin Da, calcium, phosphorus were 0 IU/kg, 0.7%, 0.45%, respectively. For treatments 3 to 5, the contents of 25-OH-Da were 50, 70 and 90 μg/kg, respectively. For treatments 6 to 8, the contents of 1a-OH-Da were 2.5, 5.0 and 10.0 μg/kg, respectively. The experiment lasted for 21 days. The results showed as follows: 1 ) compared with low calcium and phosphorus control group, except that 50 μg/kg 25-OH-D3 did not affect average daily feed intake ( P 〉 0.05 ), the supplementation of different levels of 25-OH-D3 or 10.0 μg/kg 1 a-OH-D3 significantly improved the growth performance of yellow-feath- ered broilers (P 〈 0.05) ; the supplementation of 70 and 90 μg/kg 25-OH-Da or 2.5, 5.0 and 10.0 μg/kg 1 a-OH-Da significantly improved calcium apparent and true digestibility ( P 〈 0.05 ), and the supplementation of 5.0 and 10.0μg/kg 1a-OH-D3 significantly improved phosphorus apparent and true digestibility (P 〈 0.05 ) ; the supplementation of 50, 70 and 90 μg/kg 25-OH-D3 or 10.0 μg/kg 1a-OH-D3 significantly im- proved serum phosphorus content and decreased serum the ratio of calcium to phosphorus (Ca/P) ( P 〈 0.05 ), and the supplementation of 90 μg/kg 25-OH-Da or 10.0 μg/kg 1a-OH-D3 significantly decreased ser- um tartrate resistant acid phosphatase (TRAP) activity (P 〈0.05), while there was no significant difference