在pH7.0Tris-HCl缓冲溶液中及聚乙二醇-20000存在下,羊抗兔IgG与兔IgG的免疫复合物可聚集形成疏水的免疫复合物微粒,在330,400,520nm处有三个共振散射峰,在470nm有一个同步散射峰。IgG浓度在1.33~133.3 μg·mL^-1范围内与470nnm处的散射强度呈线性。借此用于定量分析血清IgG,结果满意。方法检出限为0.99μg·mL^-1.
In pH 7.0 Tris-HCl buffer solution, goat-anti-rabbit IgG is combined with rabbit IgG specifically, and aggregates to form immune complex particles that exhibit three resonance scattering peaks at 330, 400 and 520 nm respectively, and a synchronous scattering peak at 470 nm, in the presence of PEG-20000. The scattering intensity at 470 nm is linear to the rabbit IgG concentration in the range of 1.33 to 133.3 μg·mL ^-1. The detection limit is 0.99 μg·mL^-1. The method was applied to the quantitative analysis of rabbit IgG, with satisfactory results.