中文摘要：

摘要：目的探讨二氧化氯对肠道腺病毒41型（AdV41）核酸的破坏在灭活病毒中的作用，并对病毒基因组受损区域进行定位，阐明二氧化氯灭活腺病毒的消毒规律和机制。方法在25℃，pH7．2条件下，以不同剂量（0．1、0．5、0．7、1．0、2．0mg／L）的二氧化氯对AdV41作用不同时间（0、0．5、1、2、3、4、5、7、10、20、30、60min）；采用大片段逐步步移PCR（long-overlappingPCR）分析二氧化氯对病毒全基因组的损伤，以细胞培养法检测病毒感染性。结果二氧化氯浓度变化符合幂函数Ct=a0×e^-1a规律；病毒的灭活率随着Ct值的增加而逐渐增高；当Ct=0．773mg／（L·min）时，二氧化氯对AdV41可以达到4log的灭活对数值（即灭活率为99．99％）。AdV41基因组各部分对二氧化氯的抵抗力不同，其5’的1．2081nt区域的破坏与AdV41感染性的消失一致。结论AdV41基因组5’的1-2081nt区域对二氧化氯最为敏感，且与病毒的感染性相关；在25℃，pH7．2时二氧化氯灭活4logAdV41的Ct值为0．773mg／（L·min）。

英文摘要：

Objective To evaluate the role of the destruction of viral DNA in the inactivation of adenovirus 41 by chlorine dioxide, and locate the damaged sites in viral genome, so as to provide the basis for elucidating the inactivation regularity and mechanism of adenovirus 41 by chlorine dioxide. Methods Long-overlapping polymerase chain reaction （PCR） was used to detect the damage of viral genome, and cell culture was applied to determine viral infectivity at different chlorine dioxide dosages （0.1,0.5,0.7,1.0 and 2.0 mg/L） for different contact time（0,0.5,1,2,3,4,5,7,10,20,30 and 60 rain）. Results The concentration of chlorine dioxide was consistent with the power function Ct=a0×e^-1a. The inactivation rate of virus was increased with the Ct values,being a dose-effect relationship. Ct values for 4 log inactivation at 25 ℃ was 0.773 mg/（L·min） for pH7.2. Different regions of AdV41 viral genome had different chlorine dioxide-resistance. The damage of the 1-2 081 nt of the genomic 5＇regions was in coincidence with the inactivation of AdV41. Conclusion Damage of the 1-2 081 nt of the genomic 5＇regions may cause lethal effect by chlorine dioxide. Adenovirus 41 Ct values for 4 log inactivation at 25 ℃ was 0.773 mg/（L·min） for pH7.2.