中文摘要：

目的比较水体中病毒的三种浓缩方法，评价各方法的浓缩效果，为水体中病毒浓缩方法的选择提供依据。方法将不同浓度的指示病毒（脊髓灰质炎病毒1型PV1）加入经高压灭菌处理后的污水处理厂二级出水中，采用钙离子法、牛肉汤洗膜法和载阳电荷滤料法对水样分别进行浓缩，采用逆转录-PCR技术检测灵敏度，观察指示病毒的某一特征基因片段PCR结果来确定各方法的浓缩效果，并测定回收率。结果钙离子法和载阳电荷滤料法对含最低病毒滴度的水样浓缩后均能检测出特定基因片段，钙离子法的回收率为（91．18±1．77）％，牛肉汤洗膜法的回收率为（85．49±5．59）％，滤料法的回收率为（68．63±2．45）％。结论三种方法均能浓缩水样中较低浓度的肠道病毒，钙离子法回收病毒效果最佳，牛肉汤洗膜法其次，载阳电荷滤料法回收率最低。

英文摘要：

Objective To provide a simple and efficient method for virus concentration by observing the sensitivity different concentrations and recovery of three methods for enterovirus concentration. Methods Viruses （poliovirus type 1 as an indicator virus PV1） with were added into autoclaved-sterilized secondary effluent, then the effluent was concentrated by the calcium floceulation-citrate dissolution method, the membrane adsorption-elution method and the electropositive material （poly-aluminum chloride） method, respectively. The sensitivity and recovery were evaluated by reverse transcription PCR and cell culture. Results The coefficient of recovery of the calcium flocculation-citrate dissolution method was 92.5%, and those of the methods of the membrane adsorption-elution and the membrane adsorption-elution were 86.1%, 78.3%, respectively. Conclusion The rate of recovery for the calcium flocculation-citrate dissolution method is the best, followed by the membrane adsorption-elution method and the electropositive material method.