中文摘要：

目的应用α-胞衬蛋白（α-Fodrin）免疫BALB／C小鼠，以期建立干燥综合征的实验动物模型。方法应用α-胞衬蛋白100μg（50μl）配合等体积佐剂，于0、14、35、51d进行尾根部皮下注射给药免疫4周龄的BALB／C小鼠，以颌下腺匀浆作为阳性对照，GST及PBS作为阴性对照。小鼠每周计饮水量，每2—3周取血，检测不同时间点小鼠血清中的α-胞衬蛋白抗体、M3受体蛋白多肽（M3RP）抗体、SSA抗体、SSB抗体、类风湿因子（RF）以及抗核抗体（ANA）等自身抗体，ELISA方法测定小鼠血清中的细胞因子白介素（IL）-2，IL-10和干扰素（IFN）-1。检测小鼠颌下腺病理改变及α-胞衬蛋白的抗原表达情况。结果（1）免疫前小鼠血清中的自身抗体均为阴性，而自初次免疫后35d开始，经颌下腺匀浆及α-胞衬蛋白免疫的BALB／C小鼠出现α-胞衬蛋白抗体、M3RP抗体和ANA，而上述抗体在PBS和GST对照组为阴性。（2）自初次免疫后56d开始，予颌下腺匀浆及α-胞衬蛋白免疫的BALB／C小鼠的腺体中出现少量淋巴细胞浸润，且随时间的延长，淋巴细胞浸润逐渐加重；而应用PBS和GST免疫的小鼠腺体无明显的病理改变。应用α-胞衬蛋白多抗免疫组化的方法检测颌下腺匀浆、α-胞衬蛋白免疫的BALB／C小鼠的颌下腺组织中有α-胞衬蛋白表达，而PBS和GST组为阴性。（3）IFN-γ在予α-胞衬蛋白、颌下腺匀浆、GST及PBS免疫后分别为（81．6±7．1）、（90．5±4．9）、（30．1±5．9）、（19．3±6．4）pg／ml；IL-2在上述各组免疫后分别为（18．7±2．3）、（19．8±0．9）、（4．9±1．1）、（3．5±1．6）pg／ml，与GST及PBS组相比，Ot-胞衬蛋白及颌下腺匀浆免疫可明显提高小鼠血清IFN-1和IL-2水平（P〈0．05）。（4）α-胞衬蛋白免疫对各组小鼠饮水量无明显影响。结论（1）建立了应用α-胞衬蛋白诱导的SS动物模型。（2）免疫?

英文摘要：

Objective To explore the possibility to establish Sjogren＇s syndrome models by immunizing mice with α-fodrin. Methods Twenty-four 4-week-old BALB/C mice were randomly divided into 4 equal groups to undergo subcutaneous injection of α-Fodrin, submaxillary gland homogenate and glutathione S-transferase （GST） or phosphate-buffered saline （PBS） （negative control groups） on days 0, 14, 35, and 56 respectively. The drinking amount of water was measured. Blood samples were collected every 2-3 weeks. Munofiuorescence assays and ELISA were used to examine the presence of anti-Fodrin, anti-type 3 muscarinic acetylcholine receptor polypeptide （ M3RP）, anti-SSA, anti-SSB, rheumatoid factor （RF）, and antinuclear antibody （ ANA）. Immunocbemistry was used to detect the levels of interferon （IFN）-γ , interleukin （IL）-2, and IL-10. One mouse was killed from each group every 2-3 weeks. The salivary glands were examined. Results （1） No auto-immune antibody was found in the serum samples of the mice before immunization. Antibodies against α-Fodrin and M2RP, and ANA were positive in the serum samples of the α-Fodrin and submaxillary gland homogenate groups since the 35th day after immunization, and were all negative in the 2 control groups. However, no antibodies against SSA, SSB and RF were found in all 4 groups. （2） Lymphocytic infiltration could be seen in the salivary glands of the immunized animals since 50th days after the first immunization of α-Fodrin and submaxillary gland homogenate. Immunohistochemistry showed α-Fodrin expression in the submaxillary glands of the α-Fodrin and submaxillary gland homogenate groups, but not in the PBS and GST controls. （3） The serum IFN-α levels of the α-Fodrin and submaxillary gland homogenate groups were （81.6 ± 7.1） and （90.5 ± 4.9） pg/ml respectively, both significantly higher than those of the GST and PBS groups [ （ 30. 1 ±5.9） and （ 19. 3 ± 6.4） pg/ml respectively, both P 〈 0. 05 ]. The serum IL-2 levels of th