中文摘要：

目的应用稳定表达红色荧光蛋白的人乳腺癌MDA—MB－23iSAffp细胞系，建立乳腺癌骨移植瘤动物模型，并应用活体成像技术检测肿瘤的生长情况。方法注射MDA．MB．231SArfp细胞于BALB／c雌性裸小鼠胫骨髓腔，应用活体光学成像系统，连续观察肿瘤细胞在体内的生长情况，同时测量肿瘤体积的变化；4周和7周时拍摄x线片并做组织学检查，评估肿瘤对骨组织的影响。结果利用活体光学成像系统监测发现，至第3～4周期间，肿瘤进入对数生长期；肿瘤大小与荧光信号强度呈线性正相关。X线片和组织切片显示MDA—MB．231SArfp细胞形成溶骨性的骨破坏。结论成功建立了可非侵入性监测的乳腺癌骨移植瘤模型，利用活体光学成像系统结合x线片及组织学检查能够直观、连续、准确地检测肿瘤细胞在骨环境内的生长情况，为后续抗肿瘤药物的筛选和评价提供了新的手段和工具。

英文摘要：

Objective To establish the xenograft tumor model with human breast cancer cells MDA-MB-231SArfp stably expressing red fluorescent protein （RFP） and monitor the growth of breast cancer cells in bone environment using the in vivo imaging system. Methods MDA-MB-231SArfp cells were injected intra-tibially into BALB/c-nu female nude mice. The tumor cells growth in vivo was monitored by the in vivo imaging system and tumor volume were measured every week. Seven weeks after intra-tibial inoculation, the mice were sacrificed and hind limbs were taken out for the radiographic and histological analysis. Results The results examined by the in vivo imaging system showed that the MDA-MB-231SArfp cells started rapid growth at the 3 weeks after inoculation and the changes of fluorescent intensity correlated well with the increased tumor volume. X-ray images and HE stained tissues demonstrated that MDA-MB-231SArfp cells induced more severe osteolytic lesions at the 7 weeks than 4 weeks after inoculation. Conclusions We successfuly established the non-invasive monitoring xenograft animal model using breast cancer cells labeled by the red fluorescent protein. The in vivo imaging system, combined with radiographic and histological analysis, could provide a visualized, continuous, and reliable platform to monitor the growth of breast cancer cells in bone environment, which can used to screen and evaluate the new anti-tumor drugs.