中文摘要：

利用RT-PCR和RACE技术,从新疆栽培扁桃‘鹰嘴’花药中获得了一个全长的SFB（S-hap-lotype-specific F-boxgene）基因（GenBank登录号为FJ362525）,命名为AcSFBd。该基因全长1125bp,编码374个氨基酸,在N末端含有一个大约50个氨基酸组成的F-box基序,含有两个高变区（Hva和Hvb）和两个可变区（V1和V2）。生物信息学的方法成功预测了AcSFBd蛋白分子质量为43kD,为亲水性、非分泌型蛋白,在基质内起连接酶（EC6.-.-.-）的作用。成功构建了原核表达载体,并转化大肠杆菌BL21（DE3）,SDS-PAGE检测结果显示表达了一个约60kD的蛋白。

英文摘要：

Style S-determinants have been determined to be S-RNase and the SFB （S-haplotype-specific F-box gene） alleles are considered to be the first candidates of the pollen S-determinants. A total length SFB gene （ GenBank accession No. FJ362525） named AcSFBd was cloned with RT-PCR and RACE technolgy from the anther of almond Yingzui planted in Xinjiang. AcSFBd was 1 125 bp in length encoding a protein of 374 amino acids. This gene encoded proteins with the F-box motif in the N-amino terminus, and with four （hyper） variable regions, i e. , V1, V2, Hva and Hvb which were similar with S-RNase genes, considered to be recognition site between SFB and S-RNase. With the bioinformatics analysis, the AcSFBd protein was 43 kD, was instable, hydrophilicity and non-secretory protein and had ligase enzyme activity. After transformed to Escherichia coli BL21 and induced by isopropyl-13-D-thiogalactopyranoside （IPTG） , recombinant protein about 60 kD was expressed in pET-AcSFB system and separated by SDS-PAGE electrophoresis.