兰科植物种子共生萌发需要共生真菌的参与。真菌受生境的影响,原地与异地种子萌发状况是否一致对于兰科植物保护十分重要。以华石斛Dendrobium sinense为材料,比较华石斛种子原地与实验室内播种于不同附生宿主树皮上其种子萌发情况。结果表明:(1)两者均在播种75 d后华石斛种子开始萌发,播种后135 d后附生宿主树上华石斛萌发原球茎数达到高峰,在185 d后华石斛种子萌发的原球茎数基本趋于稳定;(2)异地条件下150 d后,部分绿色原球茎开始逐渐白化消亡;(3)碎叶蒲桃上华石斛在2种环境中均有少量种子萌发,雅加松、马蹄荷上华石斛在原地与异地条件下均无种子萌发,大叶布拉栎上的华石斛只在异地萌发,竹叶松上的华石斛种子只在原地萌发。可见,原地与异地对于华石斛萌发原球茎存在一定程度的影响。
Symbiotic fungus is indispensable to seed budding of orchid plants. Habitats can affect fungus; therefore, environmental conditions in the seed germination site should be as similar as to the original habitat to keep an ideal seed germination and seedling growth. In this experiment, Dendrobium sinense seeds were germi- nated in different host barks as epiphytes in the local (the originally-grown site) site and the laboratory. The results were as follows. (1) D. sinense seeds sowed in the local site and laboratory began to germinate at day 75. The germination rate ofD. sinense protocorms grown as epiphytes on host barks peaked at day 135 and the germina- tion rate of protocorms from D.sinense seeds tended to be stabilized 185 days after seeding; (2) Under the labora- tory condition, parts of green protocorms started disappearing at day 150; (3) A small amount of D.sinense seeds planted on Syzygium buxifolium germinated under the local and laboratory conditions. There was no germination of the seed grown on Exbucklandiatonldnensi and Pinus massoniana. D. sinense seeds on Cyclobalanopsis blake germinated only under the laboratory condition, whereas D. sinense seeds on Podocarpus neriifoliu geminated only under the local condition, indicating that germination of D. sinense protocorms was influenced by the en- vironment conditions in the seeding site.