中文摘要：

钾 dehydroandrographolide succinate (DAS-K ) 有抗菌剂和抗病毒的效果。它为病毒肺病，疟疾和呼吸感染的处理广泛地被使用了。在这个工作，为 DAS-K 的决心的一个新奇流动注射化合光(CL ) 方法被建议。方法在碱的答案基于在 DAS-K 和 hexacyanoferrate (III ) 之间的反应给弱 CL 信号，它被玫瑰精 B 提高。为 CL 反应的试验性的条件被优化，可能的反应机制被讨论。在最佳条件下面， DAS-K 的集中与在 0.180 molby CV 测试的范围的 CL 紧张成正比 1.13metric 正在联合起来，被决定并且精制到 1.9 吗？？分辨率。它的 accesso 蠨?蠨 的交往的领域的结构？？

英文摘要：

Potassium dehydroandrographolide succinate （DAS-K） has antibacterial and antiviral effects. It has been used widely for the treatment of virus pneumonia, malaria and respiratory infections. In this work, a novel flow-injection chemiluminescence （CL） method for the determination of DAS-K was proposed. The method is based on the reaction between DAS-K and hexacyanoferrate（III） in alkaline solution to give weak CL signal, which is enhanced by rhodamine B. The experimental conditions for the CL reaction were optimized and the possible reaction mechanism was discussed. Under the optimum conditions, the concentration of DAS-K is proportional to the CL intensity in the range of 0.1-80 μmol·L^-1 with a detection limit of 0.05 μmol·L^-1. The interaction of the DAS-K with bovine serum albumin by on-line ultrafiltration and flow-injection chemiluminescence was studied. The concentrations of unbound DAS-K from ultra filter tube were determined by the flow-injection CL method. The binding parameters were estimated by the Scatchard plot and Klotz plot. The proposed system proved that FIA-CL coupled with on-line ultrafiltration sampling was a fast and simple technique for the study of drug-protein interaction.