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中文摘要：
了解新疆南疆地区犬伯氏疏螺旋体感染的情况及流行特点,本研究采用酶联免疫吸附试验法对从新疆南疆采集的160份犬血清进行抗伯氏疏螺旋体IgG抗体检测,比较不同来源及不同性别犬伯氏疏螺旋体抗体阳性率的差异,采用巢式PCR方法扩增伯氏疏螺旋体rrf（5S）~rrl（23S）rRNA间隔区片段进行血清样本的病原学检测。结果表明,160份被检血清中18份抗伯氏疏螺旋体IgG抗体阳性,阳性率为11.25%,其中,农村饲养犬的阳性率为21.81%（12/55）,城市家养犬的阳性率为5.71%（6/105）,差异极显著（P〈0.01）;雄性犬和雌性犬的抗体阳性率依次为11.63%（10/86）和10.81%（8/74）,差异不显著（P〉0.05）。巢式PCR检测到2份阳性样品,测序结果表明犬感染伯氏疏螺旋体属于B.garinii.基因型。
英文摘要：
In order to investigate the infection status of Borrelia burgdorferi among dogs in nanjiang region of Xinjiang,total of 160 serum samples were collected from dogs in this region,and were tested by ELISA to examine the IgG antibodies against Borrelia burgdorferi; the positive rate differences were compared by Chi-square test based on sample sources and animal genders,the 5 ~23SrRNA spacer fragments of Borrelia burgdorferi were amplified by using nested polymerase chain reaction to investigate the pathogen.The result showed that the total positive rate was 11.25%,18 out of 160 subjects were Borrelia burgdorfer IgG positive,of which 21.81%（12/55） was in rural feeding dog and 5.71%（6/105） in urban feeding dog,the difference was highly significant（P〈 0.01）; there was no difference（P 〉 0.05） between male dogs（11.63%） and female dogs（10.81%）.Two samples were tested positive by nested PCR,sequence analysis showed that the two samples were B.garinii.strains.
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