以初始体质量分别为(11.24±0.07)、(18.60±0.36)和(8.93±0.21)g的虹鳟、花鲈和大黄鱼幼鱼为研究对象,用植物油(亚麻籽油∶豆油=1∶1)分别替代0%(FO,对照组)、50%(FV)和100%(VO)的鱼油来配制3种等氮(粗蛋白含量为41%)等脂(粗脂肪含量为12%)的实验饲料。通过投喂实验探讨不同植物油替代鱼油水平对虹鳟(淡水鱼)、花鲈(广盐性鱼类)和大黄鱼(海水鱼)肝脏和肠道组织结构的影响差异。70 d投喂实验后发现,随着替代水平增加虹鳟、花鲈和大黄鱼的肝细胞中脂肪滴累积程度逐渐增高,相同替代水平下肝细胞中脂肪滴累积程度为大黄鱼〉花鲈〉虹鳟;肠道组织中杯状细胞随植物油替代水平升高而增多,而在大黄鱼全植物油组出现脂肪滴累积和上皮细胞死亡现象。以上结果表明,植物油替代鱼油对虹鳟组织结构影响最小,对大黄鱼影响最大。
A 70-day feeding trial was conducted to investigate the effects of replacement of fish oil by vegetable oil on hepatic and intestinal histology of rainbowtrout,Japanese seabass and yellowcroaker,with initial weight( 11. 24 ± 0. 07),( 18. 60 ± 0. 36) and( 8. 93 ± 0. 21) g,respectively. Three isonitrogenous( crude protein 41%) and isolipidic( crude lipid 12%) practical diets were formulated to contain graded levels of vegetable oil blend( 0%,50% and 100% dry weight) by supplementation of soybean oil and inseed oil( 1∶ 1). 0% vegetable oil blend group was treated as the control group. The three artificial diets were named FO,FV and VO,respectively. The feeding trials were conducted in indoor freshwater system( rainbowtrout) or sea floating cages( Japanese seabass and yellowcroaker). Results showed that increased lipid vacuoles in hepatocytes cytoplasm and serious intestinal inflammation was induced by increasing level of fish oil replacement in all three fish species. Among the three fishes,liver and intestinal structure of yellowcroaker was affected the most seriously by the vegetable oil.