中文摘要：

研究旨在探究饲料脂肪水平对大菱鲆（Scophthal musmaximus）幼鱼雷帕霉素受体（TOR）信号通路的影响.以大菱鲆幼鱼[初始体重（8.6±0.01）g]为实验对象,配制脂肪水平分别为11.69%（适宜脂肪组）和16.58%（高脂组）的2种等氮饲料在室内循环水系统中进行投喂实验,养殖周期为97d.结果显示：（1）饲料脂肪水平升高对大菱鲆幼鱼存活和特定生长率（SGR）无显著影响（P〉0.05）.（2）与适宜脂肪组相比,肝脏中TOR和4EBP1mRNA水平在高脂组上调（P〈0.05）而4EBP2mRNA水平在高脂组下调（P〈0.05）.此外,与适宜脂肪组相比,肌肉中TOR和4EBP1mRNA水平在高脂组下调（P〈0.05）而4EBP2mRNA水平在高脂组无显著变化.（3）饲料脂肪水平显著影响AKT、TOR和4EBPs磷酸化水平.肝脏中p-AKT（T308和S473）/AKT、p-mTOR（S2448）/mTOR和p-4EBP1（T37/46）/4EBP1在高脂组显著高于适宜脂肪组,而p-p70S6K（T389）/p70S6K在高脂组显著低于适宜脂肪组.肌肉中p-AKT（S473）/AKT、p-mTOR（S2448）/mTOR和p-4EBP1（T37/46）/4EBP1在高脂组显著低于适宜脂肪组,而p-p70S6K（T389）/p70S6K在高脂组显著高于适宜脂肪组.结果表明：大菱鲆幼鱼肝脏和肌肉TOR、4EBP1和4EBP2的mRNA表达量与AKT、TOR、4EBP1和p70S6K磷酸化受到饲料脂肪水平调控,饲料脂肪水平升高,激活了肝脏TOR信号通路,同时肌肉TOR信号通路受到部分抑制.

英文摘要：

This study investigated the effects of dietary lipid levels on the TOR pathway in juvenile turbot using dietscontaining graded levels of lipid 11.69% （L11.69） and 16.58% （L16.58） （dry weight） to feed triple groups of juvenileturbot （8.6±0.01 g） for a 97d trial. The lipids level in diet did not impact survival rate and SGR. Compared those inL11.69 group, L16.58 group increased the gene expression of TOR, 4EBP1 but decreased 4EBP2 in liver, and it in-creased the phosphorylation of pAKT-S473, pAKT-T308, pmTOR-S2448, and p4EBP1-T37/46. In the muscle, L16.58group had decreased TOR and 4EBP1 associated with pAKT-S473, pmTOR-S2448 and p4EBP1-T37/46 comparedwith those in L11.69 group. In conclusion, L16.58 diet can activate TOR pathway in liver while partially suppress TORpathway in muscle of junvenile turbot compare with L11.69 diet.