一个12周的饲养试验进行评估膳食水苏糖对生长性能的影响,消化酶活性和肠道结构的少年多宝鱼(大菱鲆)。五等氮(粗蛋白49.58%)和isolipidic(粗脂肪10.50%)日粮配方含有0(对照),0.625%(s-0.625),1.25%(s-1.25),2.5%(s-2.5)和5%(S-5)水苏糖,分别。随着水苏糖水平升高,生长性能和饲料利用率等多宝鱼,特定生长率,最终的平均体重,增重率和饲料效率,显著增加(P<0.05),然后稳定。鱼喂S-5采食量显著高(P<0.05)比其他组的鱼。活动关系的胰蛋白酶,肠酪蛋白,胃和肠道淀粉酶的水苏糖的显著影响(P<0.05)。胰蛋白酶和肠酪蛋白的活动的最高值是在s-1.25观察组,对照组以观察胃淀粉酶活性最高,肠淀粉酶活性最低。没有病变或损伤在所有处理鱼的远端小肠结构,而在远端小肠简单褶皱高度显著增加(P<0.05)时,1.25%或2.5%是在日粮中添加水苏糖。这些结果表明,中等水平的水苏糖(1.25%)改善生长性能,饲料利用率,消化酶活性和少年多宝鱼远端小肠结构。
A 12-week feeding trial was conducted to evaluate the effects of dietary stachyose on the growth performance, digestive enzymes activities and intestinal structures of juvenile turbot (Scophthalmus maximus L). Five isonitrogenous (49.58% crude protein) and isolipidic (10.50% crude lipid) diets were formulated to contain 0 (Control), 0.625% (S-0.625), 1.25% (S-1.25), 2.5% (S-2.5) and 5% (S-5) stachyose, respectively. With the increase of stachyose level, the growth performance and feed utilization of turbot, such as the specific growth rate, final mean body weight, weight gain rate and feed efficiency, increased significantly (P〈0.05) and then stabilized. The feed intake of fish fed S-5 was significantly higher (P〈 0.05) than that of fish in other groups. The activi- ties of trypsin, intestinal caseinolytic, stomach and intestinal amylase were significantly influenced by stachyose (P〈0.05). The highest values of trypsin and intestinal caseinolytic activities were observed in group S-1.25, while the highest activity of stomach amylase and the lowest activity of intestine amylase were observed in group S-5. No lesion or damage was found on the distal intestine structures of fish from all treatments, while the height of simple folds in the distal intestine was significantly increased (P 〈0.05) when 1.25% or 2.5% stachyose was added in the diets. These results indicated that moderate level of stachyose (1.25%) improves the growth performance, feed utilization, digestive enzyme activities and the distal intestine structures of juvenile turbot.