中文摘要：

对光谱法在分子间非共价相互作用中的应用作了较详细的评述。重点介绍了紫外、荧光、圆二色、红外、激光拉曼、共振散射等方法在分子间相互作用研究中的应用及进展,总结了这些方法的优缺点。紫外-可见吸收光谱简便、快速,荧光光谱法可获得分子间作用的结合常数、结合位点数及作用方式等信息,圆二色谱法是测定作用前后生物大分子构象变化的有效方法,红外光谱法可提供蛋白质二级结构及构象变化的谱学信息,拉曼光谱法是研究溶液中生物大分子构象变化的有效技术,表面等离子共振（SPR）技术能在保持生物分子天然活性的条件下实时检测分子间的相互作用。

英文摘要：

Spectrophotometric method is widely used in the structure determination of biologic macromolecules and non-covalent interactions study for its convenience and speed.In the present paper,spectroscopy methodologies in the study of non-covalent interactions between small-molecule and biomacromolecule is comprehensively reviewed with 25 references.This review article focuses on the applications and development of common spectroscopy methodologies in the study of non-covalent interactions between small molecule and biomacromolecule,including the UV,fluorescence,CD,IR,Raman,resonance light scattering technique and SPR.The advantages and disadvantages of spectroscopy methodologies are also described.UV-Vis absorption spectrum（UV） method is widely used in the study of non-covalent interactions for its convenience and speed.The binding site number,the apparent binding constant and the interaction mode of non-covalent interactions can be obtained by fluorescence spectrum method.Circular dichroism（CD） method is effective way in the study of non-covalent interactions measure.Spectroscopy information about protein secondary structure and conformation can be acquired by infrared spectrometry（IR） method.Raman spectroscopy method is a better way to investigate the conformation change in macromolecules in solution.Non-covalent interactions can be measured by surface plasma resonance（SPR） method under the natural active condition.X-ray diffraction analysis method is better for non-covalent interactions research,but it is difficult to cultivate crystalline complex.