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中文摘要：
以溴化乙锭（EB）为荧光探针，对色氨酸二肽（Trp-Trp）与小牛胸腺DNA的作用机制进行了研究，考查了酸度、离子强度及磷酸根离子对Trp-Trp与DNA相互作用的影响；结果表明，在pH7．2的模拟体液条件下，Trp-Trp可与DNA发生相互作用；向DNA-EB体系中加入Trp-Trp时荧光逐渐减弱，这说明Trp-Trp能与DNA发生作用。通过Scatchard方程进一步分析发现，Trp-Trp对DNA—EB的影响表现为混合模式：一方面，Trp-Trp能中和DNA上的磷氧负离子，导致DNA链收缩，从而影响DNA的构象，使嵌入的EB从DNA中部分游离出来，荧光减弱；另一方面，Trp-Trp与EB也存在静电位点竞争。
英文摘要：
The interaction between tryptophan dipeptide （Trp-Trp） and ct-DNA was studied by fluorescence spectroscopy using ethidium bromide （EB） as fluorescence probe, the effect factors such as acidity, salt effect and phosphoric acid ion on the interaction are investigated; It is showed that in Tris-HCl （pH 7.2）, Trp-Trp can react with DNA. The fluorescence intensity of DNA-EB quenched with the increase of Trp-Trp. According to the equation of scatchard, influence of Trp-Trp on interaction of DNA-EB belongs to mixed model. On the one hand, the oxyphosphate anion of DNA can be neutralized by Trp-Trp partially, which induced the contraction of DNA and the conformational change of DNA, leading to release of EB from DNA partially; on the other hand, the Trp-Trp can also compete the electrostatic sites with EB directly.
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ESI-MS studies on novel liquid homo-peptide libraries and their conjugate libraries directed by phos
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