中文摘要：

利用富集培养的方法从南昌市郊某养鱼塘采样分离出22株反硝化细菌,其中8株反硝化率较高,从中选择一株效果最好的作为研究对象,命名为HS-N62,对其生长特性进行了深入研究。结果表明：硝酸盐氮初始浓度为140mg/L,菌株HS-N62在12h内对硝酸盐氮的去除率可达96%,而且没有亚硝酸盐氮的积累。该菌最适生长温度范围为30°C-37°C,最适生长pH范围6.0-8.0,最适C/N比为10：1,并能利用多种碳源生长。运用正交试验探讨了该菌株最适的反硝化条件。反硝化菌株HS-N62还具有较好的除磷能力,12h除磷率达到67.7%（初始磷酸盐浓度57mg/L）。通过形态学特性和生理生化分析以及16S rRNA基因序列分析,菌株HS-N62与Pseudomonas sp.亲缘关系最为接近,相似性达99%,初步鉴定该菌为假单胞菌属（Pseudomonas sp.）。

英文摘要：

Twenty two bacterial strains with a capacity of denitrification were isolated from fish pond,of which eight strains have the high rate of denitrification while one strain named HS-N62 has the strongest effect of denitrification.The results showed that the denitrifying rate of strain HS-N62 reached 96% in 12 hours with initial nitrate nitrogen concentration of 140 mg/L and no nitrite nitrogen accumulation.The growth rate and characteristics of HS-N62 were further studied,and the results showed that the optimum growth temperature ranged from 30°C to 37°C,pH value from 6.0 to 8.0 and the optimal C/N ratio 10：1.Meanwhile strain HS-N62 could utilize a variety of carbon sources for growth.The most suitable denitrification condition for strain HS-N62 was confirmed by using a series of the orthogonal test.This denitrifying strain HS-N62 also has good ability of phosphorus removal,and the removal rate within 12 hours was up to 67.7%（at initial phosphate concentration 57 mg/L）.Strain HS-N62 showed 99% 16S rRNA gene sequence similarity to many Pseudomonas strains.Based on the morphological characteristics,physiological and biochemical characteristics,the strain HS-N62 was identified as Pseudomonas sp.