中文摘要：

为了建立一种便捷、灵敏的犬流感病毒（cⅣ）检测技术，本研究根据CTV相对保守的M片段设计3对特异性引物，建立CIV的RT—LAMP检测方法，对反应体系中的MgS04、Betaine、Bst DNA PoLymerase、dNTP、引物浓度等分别进行了优化，并进行敏感性和特异性试验。结果表明：所建立的反应体系在恒温水浴锅中作用45min即可得到其特有的阶梯状条带，而且对犬细小病毒、犬瘟病毒和犬副流感病毒的扩增结果均为阴性。该方法对CIVRNA的最小检测限为0．1pg，灵敏性高于一步RT—PCR方法。RT-LAMP和普通RT-PCR方法检测临床样品的符合率为93．02％。该方法为犬流感病毒的临床检测提供了一种简便、实用的方法，为基层检疫提供了方便。

英文摘要：

In this paper, a simple, quick RT-LAMP method was established to detect canine influenza virus （C1V） using specific outer, inner and loop primers targeted the M gene conserved regions of CIV. The results showed that the RT-LAMP method developed in this experiment with a 0.1 pg detection limit of CIV RNA, was more sensitive than RT-PCR method and of high specificity, which could be easily adapted for detection of CIV.