中文摘要：

目的利用荧光光谱法研究Eu3＋-美他环素（MTC）-溴化十六烷基三甲铵（CTMAB）体系的稀土发光效应和最佳形成条件，并对其作用机制进行探讨。方法建立测定MTC的荧光分光光度法，探讨在最佳实验条件下不同浓度的MTC和MTC配合物分别对大肠杆菌和金黄色葡萄球菌的抑菌效果。结果实验结果表明，在pH9．0—10．5Na2B4O7-NaOH的缓冲溶液中，美他环素和阳离子表面活性剂溴化十六烷基三甲铵对Eu3＋的荧光具有显著协同增敏作用，当MTC浓度为0．45—12ug·mL-1时，荧光强度与浓度呈良好的线性关系。结论据此建立了测定药物制剂中美他环素含量的荧光光谱法，方法的检出限0．0153ug·mL-1，标准偏差1．03％，不同浓度的美他环素和美他环素配合物对大肠杆菌抑菌效果较好，当美他环素浓度为1×10^-3mol·L-1时对大肠杆菌抑菌效果最好。

英文摘要：

OBJECTIVE To study the luminescence effect of Eu3 ＋ -Metacycline （MTC） -cetyltrimethyl ammonium bromide （ CT- MAB） system and the luminescence mechanism of the system. METHODS Based on the luminescence effect, a fluorescent method for the determination of MTC was proposed. Fluorescence characteristics of the complex of metacycline （MTC） with Eu3＋ in a micellar system was studied, and the effect of different concentrations of MTC and MTC complexes on Escherichia coli and the Staphylococcus aureus in optimum experimental conditions was investigated. RESULTS The experiments indicated that in pH 9.0 - 10. 5 Na2B4O7- NaOH buffer solution, both MTC and cationic surfactant （CTMAB） could enhance greatly the fluorescence intensity of Eu3＋. The fluorescence intensity was linearly related to the fleroxacin concentration in the range of 0.45 - 12 ug . mL-1. CONCLUSION A new fluorescent method has been developed for the determination of MTC in pharmaceutical preparations. The detection limit was 0. 015 3 ug .mL-1 and the RSD was 1.03%. The complexes of different concentrations of MTC and MTC have good antibacterial effect against Escherichia coli, and the antibacterial effect is the best when the MTC＇s concentration is 1 × 10^-3mol . L-1 against escherichia colon.