中文摘要：

目的：在大肠杆菌中研究透膜小肽抑制α-Synuclein（Α53T,S129Α,WT）的异常聚集。方法：基于α-Syn核心区的疏水区域设计了5种包含7个精氨酸的多聚精氨酸多肽膜透过传输系统的小肽R7P1～R7P5;构建融合蛋白α-Syn-GFP,其中α-Syn基因融合于GFP上游,透膜小肽以双顺反子方式与α-Syn-GFP共表达;以文献报道的可抑制α-Syn异常聚集的小肽ASI1D（MRRGGAVVTG（R）6）为对照,通过监测整体细胞荧光强度研究透膜小肽影响α-Syn（Α53T,S129Α,WT）的异常聚集的情况。结果：5种小肽能不同程度的抑制α-Syn（Α53T,S129Α,WT）的异常聚集;和对照Pc相比,R7P4抑制α-Syn（A53T）异常聚集的效果提高了55%,R7P3抑制α-Syn（S129A）异常聚集的效果提高了64%,R7P4抑制α-Syn（WT）异常聚集的效果提高了39%。结论：在大肠杆菌中,透膜小肽可以有效地抑制α-Syn（Α53T,S129Α,WT）的异常聚集。

英文摘要：

Objective：Study aggregation inhibition of α-Synuclein（Α53T,S129Α,WT） with membrane permeational peptides in E.coli.Method：α-Syn were fussed upstream to GFP to create of a fusion constructs of α-SynG,five membrane permeational peptides R7P1～R7P5 which comes from the hydrophobic region of α-Syn was co-expressed with α-Syn-GFP as a bicistronic.Using the small peptide ASI1D（MRRGGAVVTG（R）6） as control,the ability of the five membrane permeational peptides to aggregation inhibition of α-Syn（A53T,S129A,WT） in E.coli was studied by monitor the whole-cell fluorescence intensity.Result：Five membrane permeational peptides can inhibition the aggregation of α-Syn （A53T,S129A,WT）.Compared with ASI1D ,for α-Syn （A53T） R7P4 can increase the inhibitory effect by 55%,and for α-Syn （A53T） R7P4 can increase the inhibitory effect by 64%,for α-Syn （A53T） R7P3 can increase the inhibitory effect by 39%.Conclusion：The membrane permeational peptides can inhibition the aggregation of α-Syn （Α53T,S129A,WT） in E.coli.