中文摘要：

客观：调查 RNAi 的禁止的效果(RNA 干扰， RNAi ) 在前列腺癌房间的 CXCR4 表示上的表示向量排队。方法：为 CXCR4 基因的 Smallinterference RNA (siRNA ) 表示向量被构造，进前列腺癌房间的 transfected 排队(PC-3m 和 LNCaP ) 与 lipo 一些。CXCR4 的表示被 RT-PCR 和西方的污点检测。结果：在 PC-3m 和 LNCaPcells 的 CXCR4 mRNA 和蛋白质的表示被 RNAi 表示向量减少。在 thePC-3m 房间的 CXCR4 mRNA 表示的禁止的率是 87.81% ± 10.20% ，在第 24 小时的 56.10%± 9 .32% 和第 48 小时，比较 with56.93%± 8 .78% ， 49.24% ± 11.23% 在 LNCaP 房间。CXCR4protein 的表示的禁止的率是 64.71%± 6 .68% ， 58.66%± 1 1.56% 分别地。结论：CXCR4 基因的表示能被 RNAi 表示向量有效地禁止。

英文摘要：

Objective： To investigate the inhibitory effects of RNAi （ RNA interference, RNAi） expression vector on CXCR4 expression in prostate carcinoma cell lines. Methods： Small interference RNA （siRNA） expression vectors for CXCR4 gene were constructed and transfected into prostate carcinoma cell lines（PC-3m and LNCaP）with liposomes. T expression of CXCR4 was detected by RT-PCR and western blot. Results： T expression of CXCR4 mRNA and protein in the PC-3m and LNCaP cells was reduced by RNAi expression vectors. The inhibitory rate of CXCR4 mRNA expression in the PC-3m cells was 87.81% ± 10.20% ,56.10% ± 9.32% at the 24th hour and the 48th hour, compared with 56.93% ±8.78% ,49.24% ± 11.23% in LNCaP cells. The inhibitory rate of the expression of CXCR4 protein was 64.71% ± 6.68% ,58.66% ± 11.56% respectively. Conclusion： The expression of CXCR4 gene can effectively be inhibited by RNAi expression vectors.