中文摘要：

为探讨维拉帕米对低温诱发的肉鸡肺血管重塑的影响，动态观察了肺小动脉中膜平滑肌的增殖及管腔面积的改变。150只AA商品代肉仔鸡常规育雏，15日龄随机分为三组：常温（22～24℃）对照组、低温（9～11℃）组、低温维拉帕米组。分别于低温处理后2周（28日龄）、3周（36日龄）、4周（44日龄）从三组随机各抽取10只测定肺动脉压（PAP），并取肺组织做石蜡切片，以Weigert弹力纤维染色，计算机图象分析法测定肺细小动脉外径和内径、管总面积和管腔面积，计算相对中膜厚度（mMTPA）及管壁面积／管总面积WA／TA。结果显示：（1）维拉帕米抑制低温肉鸡肺动脉压的升高，28日龄、36日龄和44日龄，维拉帕米mPAP显著低于低温组（P〈0．05）；（2）28日龄、36日龄和44日龄时，维拉帕米组50~100lμm的肺小动脉相对中膜厚度mMTPA显著低于低温组（P〈0．05），100～200岬肺小动脉的组间差异与此类似，维拉帕米能抑制肺小动脉中膜平滑肌增生；（3）3个日龄的维拉帕米组肺小动脉管壁面积／管总面积WA／TA显著低于低温组（P〈0．05），维拉帕米能抑制血管壁的增厚。这些结果提示维拉帕米能抑制低温诱发的肺动脉高压和以肺小动脉中膜平滑肌增生、管壁增厚为特征的肺血管重塑。

英文摘要：

The effect of Verapamil, a calcium antagonist, on pulmonary vascular structural remodeling induced by low ambient temperature was investigated through observing the dynamic change of pulmonary arterioles medial thickness and pulmonary blood vessel endometry area caused by proliferous smooth muscle cells in broiler chickens. One hundred and fifty 15-day old commercial broiler chickens were randomly divided into 3 groups as follows： 50 birds were exposed to normal temperature （22-2g℃）, 50 birds were exposed to low temperature （9-11℃）, and 50 birds were exposed to low temperature （9-11℃） and received 0.01% Verapamil in drinking water. At 28, 36 and 44 days of age,10 birds were randomly taked from each group.Pulmonaryarterial pressure （PAP） was determined as index of pulmonary hypertension. The measurement of mean medial thickness in pulmonary arterioles （mMTPA） and ratio of vessel wall area to vessel total area （WA/TA） were conducted by using the microscopic image analysis program. The results showed as follows： （1）Verapamil significantly inhibited the increase of mPAP（kPa）, Verapamil was lower than low temperature group at 28, 36 and 44 days （P〈0.05）; （2） at 28, 36 and 44 days of age, the mMTPA of 50-100 μm pulmonary arterioles of low temperature group was obviously higher than that of Verapamil group （P〈0.05）, it bears an analogy to the differences between groups in 100-200 μm of pulmo muscular prolieration of pulmonary arterioles; （3） the nary arterioles, Verapamil could inhibit the medial smooth WA/TA of pulmonary arterioles of low temperature group was obviously higher than that of Verapamil group （P〈0.05）, Verapamil inhibited the hypertrophy of the vessel wall. In summary, the results indicated that Verapamil could inhibit pulmonary vascular structural remodeling and pulmonary artery hypertension induced by low ambient temoerature in broiler chickens.