中文摘要：

为探讨钙信号与犬肾上皮细胞（MDCK）活力之间的关系，采用MTT比色法观察了特异性钙调素拮抗剂-三氟拉嗪，胞内Ca^2＋螯合剂-BAPTA／AM以及Ca^2＋载体-A23187对MDCK细胞活力的影响。结果表明，12．5,25μmol／L三氟拉嗪和15μmol／L的BAPTA／AM作用72h后分别使MDCK细胞活力降至对照组的51．4％，25．1％和34％，三氟拉嗪与BAPTA／AM对MDCK细胞活力的抑制作用随药物浓度增加和作用时间延长而增强；5μmol／L的A23187对MDCK细胞具有一定程度的刺激效应，但这种刺激效应随时间的延长而减弱；15μmol／L的BAPTA／AM可增强三氟拉嗪对MDCK细胞活力的抑制作用。说明Ca^2＋-钙调素系统在MDCK细胞生长和增殖过程中发挥重要作用。

英文摘要：

The objective of this study was to explore the relationship between calcium signals and viability of Mardin-Darby canine kidney （MDCK） cells in vitro. MTT colorimetric assay were used to detect the effects of specific calmodulin antagonists, trifluoperazine （TFP）, as well as Ca^2＋ chelators, BAPTA/AM and calcium ionophore A23187 on the viability of MDCK cells in vitro. The results showed that 12.5 μmol/L TFP,25 μmol/L TFP and 15 /μmol/L BAPTA/AM obviously decreased the viability of MDCK cells to 51.4% ,25.1% and 34% of the control after 72 hours, respectively. TFP and BAPTA/AM inhibited the viability of MDCK cells in a dose-and or time-dependent manner, the higher concentration and the longer time, the more significant the inhibition effects. A23187 （5 μmol/ L） stimulated the cell viability in a certain extent,but the stimulatory effect was decreased with prolonged time. The presence of 15 μmol/L BAPTA/AM obviously enhanced the inhibition of TFP on the viability of the MDCK cells. The experimental findings presented here suggest that Ca^2＋ CaM system may be engaged in in the development and proliferation of MDCK cells to modulate the cell viability and behavior.